ArticleThe EMBO journal2000
Phosphorylation status of the SCR homeodomain determines its functional activity: essential role for protein phosphatase 2A,B'.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 66 citations in OpenAlex.
- AKR1B10 dictates c-Myc stability to suppress colorectal cancer metastasis via PP2A nitration.Science advances · 2025Article
- Dynamics of protein synthesis in the initial steps of strobilation in the model cestode parasite Mesocestoides corti (syn. vogae).Journal of proteomics · 2020Article
- Post-translational modifications of Drosophila melanogaster HOX protein, Sex combs reduced.PloS one · 2020Article
- Kinase Regulation of HOX Transcription Factors.Cancers · 2019Review
- Hoxa13 regulates expression of common Hox target genes involved in cartilage development to coordinate the expansion of the autopodal anlage.Development, growth & differentiation · 2019Article
- Protein phosphatases in chromatin structure and function.Biochimica et biophysica acta. Molecular cell research · 2019Review
- Flexibility and Disorder in Gene Regulation: LacI/GalR and Hox Proteins.The Journal of biological chemistry · 2015Review
- Acquisition of a leucine zipper motif as a mechanism of antimorphy for an allele of the Drosophila Hox gene Sex combs reduced.G3 (Bethesda, Md.) · 2014Article
- Developmental competence and the induction of ectopic proboscises in Drosophila melanogaster.Development genes and evolution · 2013Article
- Phosphatase: PP2A structural importance, regulation and its aberrant expression in cancer.Cancer letters · 2013Review
- Sequential phosphorylation of smoothened transduces graded hedgehog signaling.Science signaling · 2011Article
- Functions of B56-containing PP2As in major developmental and cancer signaling pathways.Life sciences · 2010Review
- Surprising flexibility in a conserved Hox transcription factor over 550 million years of evolution.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Phosphorylation of HOX11/TLX1 on Threonine-247 during mitosis modulates expression of cyclin B1.Molecular cancer · 2010Article
- Quantitative study of synthetic Hox transcription factor-DNA interactions in live cells.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Function and specificity of synthetic Hox transcription factors in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Article
- Hox specificity unique roles for cofactors and collaborators.Current topics in developmental biology · 2009Review
- Context-dependent regulation of Hox protein functions by CK2 phosphorylation sites.Development genes and evolution · 2008Article
- Protein kinase C-mediated phosphorylation of the leukemia-associated HOXA9 protein impairs its DNA binding ability and induces myeloid differentiation.Molecular and cellular biology · 2004Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sex combs reduced (SCR) is a Drosophila Hox protein that determines the identity of the labial and prothoracic segments. In search of factors that might associate with SCR to control its activity and/or specificity, we performed a yeast two-hybrid screen. A Drosophila homologue of the regulatory subunit (B'/PR61) of serine-threonine protein phosphatase 2A (dPP2A,B') specifically interacted with the SCR homeodomain. The N-terminal arm within the SCR homeodomain was shown to be a target of phosphorylation/dephosphorylation by cAMP-dependent protein kinase A and protein phosphatase 2A, respectively. In vivo analyses revealed that mutant forms of SCR mimicking constitutively dephosphorylated or phosphorylated states of the homeodomain were active or inactive, respectively. Inactivity of the phosphorylated mimic form was attributed to impaired DNA binding. Specific ablation of dPP2A,B' gene activity by double-stranded RNA-mediated genetic interference resulted in embryos without salivary glands, an SCR null phenotype. Our data demonstrate an essential role for Drosophila PP2A,B' in positively modulating SCR function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.