ArticleThe EMBO journal2000
Peptide antagonists of the plasmodesmal macromolecular trafficking pathway.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 80 citations in OpenAlex.
- Distributing Plant Developmental Regulatory Proteins via Plasmodesmata.Plants (Basel, Switzerland) · 2024Review
- Function of Plasmodesmata in the Interaction of Plants with Microbes and Viruses.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Small RNA Functions as a Trafficking Effector in Plant Immunity.International journal of molecular sciences · 2019Review
- Identification of evolutionarily conserved amino acid residues in homeodomain of KNOX proteins for intercellular trafficking.Plant signaling & behavior · 2014Article
- The chaperonin CCT8 facilitates spread of tobamovirus infection.Plant signaling & behavior · 2012Article
- Dissecting plasmodesmata molecular composition by mass spectrometry-based proteomics.Frontiers in plant science · 2012Article
- A plasmodesmata-localized protein mediates crosstalk between cell-to-cell communication and innate immunity in Arabidopsis.The Plant cell · 2011Article
- Opportunities and successes in the search for plasmodesmal proteins.Protoplasma · 2011Review
- Cell-to-cell trafficking of RNA and RNA silencing through plasmodesmata.Protoplasma · 2011Review
- Plasmodesmal receptor-like kinases identified through analysis of rice cell wall extracted proteins.Protoplasma · 2011Article
- Information processing without brains--the power of intercellular regulators in plants.Development (Cambridge, England) · 2010Review
- MPB2C, a microtubule-associated protein, regulates non-cell-autonomy of the homeodomain protein KNOTTED1.The Plant cell · 2007Article
- Plasmodesmal-associated protein kinase in tobacco and Arabidopsis recognizes a subset of non-cell-autonomous proteins.The Plant cell · 2005Article
- Plant and animal homeodomains use convergent mechanisms for intercellular transfer.EMBO reports · 2005Article
- A novel cell-to-cell trafficking assay indicates that the KNOX homeodomain is necessary and sufficient for intercellular protein and mRNA trafficking.Genes & development · 2005Article
- Different subcellular localization and trafficking properties of KNOX class 1 homeodomain proteins from rice.Plant molecular biology · 2004Article
- A subclass of plant heat shock cognate 70 chaperones carries a motif that facilitates trafficking through plasmodesmata.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- A novel plant homeodomain protein interacts in a functionally relevant manner with a virus movement protein.Plant physiology · 2002Article
- Intercellular trafficking of a KNOTTED1 green fluorescent protein fusion in the leaf and shoot meristem of Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Plasmodesmata: pathways for protein and ribonucleoprotein signaling.The Plant cell · 2002Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plants, cell-to-cell transport of endogenous and viral proteins and ribonucleoprotein complexes (RNPCs) occurs via plasmodesmata. Specificity of this transport pathway appears to involve interaction between such proteins/RNPCs and plasmodesmal chaperones/receptors. Here, KN1 and the cucumber mosaic virus movement protein (CMV-MP) were used, in a modified phage-display screening system, to identify peptides capable of interacting with proteins present in a plasmodesmal-enriched cell wall fraction. Binding/competition assays and microinjection experiments revealed that these phage-displayed peptides and homologous synthetic oligopeptides function as ligand-specific antagonists of macromolecular trafficking through plasmodesmata. A KN1 peptide antagonist had the capacity to interact with a motif involved in the dilation of plasmodesmal microchannels. Although KN1 could still achieve limited movement through plasmodesmata when this SEL motif was blocked, KN1-mediated transport of KN1-sense RNA was fully inhibited. These findings provide direct support for the hypothesis that KN1 requires, minimally, two physically separated signal motifs involved in the dilation of, and protein translocation through, plasmodesmal microchannels, and provide direct proof that plasmodesmal dilation is a prerequisite for the cell-to-cell transport of an RNPC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.