ArticleThe Journal of physiology2000
Disruption of actin impedes transmitter release in snake motor terminals.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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Who cites it
36 citing papers in PubMed, 86 citations in OpenAlex.
- Deciphering the Nanoscale Architecture of Presynaptic Actin Using a Micropatterned Presynapse-on-Glass Model.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Exploring the Role of RhoA/ROCK Signaling in Pain: A Narrative Review.Aging and disease · 2025Review
- Single-vesicle imaging reveals actin-dependent spatial restriction of vesicles at the active zone, essential for sustained transmission.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Actomyosin-mediated inhibition of synaptic vesicle release under CBTranslational psychiatry · 2024Article
- Presynapses contain distinct actin nanostructures.The Journal of cell biology · 2023Article
- Presynaptic Rac1 controls synaptic strength through the regulation of synaptic vesicle priming.eLife · 2022Article
- Review
- Extracellular matrix protein laminin β1 regulates pain sensitivity and anxiodepression-like behaviors in mice.The Journal of clinical investigation · 2021Article
- Article
- Microtubule and Actin Differentially Regulate Synaptic Vesicle Cycling to Maintain High-Frequency Neurotransmission.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- The actin binding protein scinderin acts in PC12 cells to tether dense-core vesicles prior to secretion.Molecular and cellular neurosciences · 2017Article
- Neuronal Porosome Complex: Secretory Machinery at the Nerve Terminal.Discoveries (Craiova, Romania) · 2017Review
- Actin Filaments Regulate Exocytosis at the Hair Cell Ribbon Synapse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- The neuronal porosome complex in health and disease.Experimental biology and medicine (Maywood, N.J.) · 2016Review
- Article
- "Late" macroendosomes and acidic endosomes in vertebrate motor nerve terminals.The Journal of comparative neurology · 2012Article
- The F-actin cortex in chromaffin granule dynamics and fusion: a minireview.Journal of molecular neuroscience : MN · 2012Review
- F-actin-myosin II inhibitors affect chromaffin granule plasma membrane distance and fusion kinetics by retraction of the cytoskeletal cortex.Journal of molecular neuroscience : MN · 2012Article
- Dual pools of actin at presynaptic terminals.Journal of neurophysiology · 2012Article
- Actin- and dynamin-dependent maturation of bulk endocytosis restores neurotransmission following synaptic depletion.PloS one · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
To investigate the role of actin in vertebrate nerve terminals, nerve-muscle preparations from garter snake (Thamnophis sirtalis) were treated with the actin-depolymerizing agent latrunculin A. Immunostaining revealed that actin filaments within presynaptic motor terminal boutons were disrupted by the drug. In preparations loaded with the optical probe FM1-43, destaining was reduced by latrunculin treatment, suggesting that transmitter release was partially blocked. Latrunculin treatment did not influence the amplitude or time course of spontaneous miniature endplate potentials (MEPPs). Similarly, endplate potentials (EPPs) evoked at low frequency were comparable in control and latrunculin-treated curarized preparations. Brief tetanic stimulation of the muscle nerve (25 Hz, 90 s) depressed EPP amplitudes in both control and latrunculin-treated preparations. After tetanus, EPPs elicited at 0. 2 Hz in control preparations recovered rapidly (0-5 min) and completely (usually potentiating to above pre-tetanus levels; 130 +/- 11 %, mean +/- s.e.m.). In contrast, EPPs evoked in latrunculin-treated preparations recovered slowly (8-10 min) and incompletely (84 +/- 8 %). The influence of latrunculin on post-tetanic EPPs depended on its concentration in the bath (KD = 3. 1 microM) and on time of incubation. These observations argue that actin filaments facilitate transmitter release rather than impede it. Specifically, actin may facilitate mobilization of vesicles towards the releasable pools.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.