ArticleProceedings of the National Academy of Sciences of the United States of America2000
The C-terminal domain-phosphorylated IIO form of RNA polymerase II is associated with the transcription repressor NC2 (Dr1/DRAP1) and is required for transcription activation in human nuclear extracts.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
- A host susceptibility gene, DR1, facilitates influenza A virus replication by suppressing host innate immunity and enhancing viral RNA replication.Journal of virology · 2015Article
- Fibrillarin methylates H2A in RNA polymerase I trans-active promoters in Brassica oleracea.Frontiers in plant science · 2015Article
- Taspase1 processing alters TFIIA cofactor properties in the regulation of TFIID.Transcription · 2015Article
- RNA polymerase II conserved protein domains as platforms for protein-protein interactions.Transcription · 2011Article
- Dr1 (NC2) is present at tRNA genes and represses their transcription in human cells.Nucleic acids research · 2010Article
- Hypoxic repression of endothelial nitric-oxide synthase transcription is coupled with eviction of promoter histones.The Journal of biological chemistry · 2010Article
- Regulation of nuclear import and export of negative cofactor 2.The Journal of biological chemistry · 2009Article
- TFIIB recognition elements control the TFIIA-NC2 axis in transcriptional regulation.Molecular and cellular biology · 2009Article
- The dual control of TFIIB recruitment by NC2 is gene specific.Nucleic acids research · 2008Article
- Human hHpr1/p84/Thoc1 regulates transcriptional elongation and physically links RNA polymerase II and RNA processing factors.Molecular and cellular biology · 2005Article
- Multi-protein complexes in eukaryotic gene transcription.Plant molecular biology · 2002Review
- Direct stimulation of transcription by negative cofactor 2 (NC2) through TATA-binding protein (TBP).Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Retinoic acid receptors inhibit AP1 activation by regulating extracellular signal-regulated kinase and CBP recruitment to an AP1-responsive promoter.Molecular and cellular biology · 2002Article
- Yeast NC2 associates with the RNA polymerase II preinitiation complex and selectively affects transcription in vivo.Molecular and cellular biology · 2001Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Activation of class II gene transcription may involve alleviation of transcription repression as well as stimulation of the assembly and function of the general RNA polymerase (RNAP) II transcription machinery. Here, we investigated whether activator-reversible transcription repression by NC2 (Dr1/DRAP1) contributes to maximum induction levels in unfractionated HeLa nuclear extracts. Surprisingly, we found that depletion of NC2 does not significantly affect basal transcription, but dramatically reduces activated transcription. Immunoblot analyses revealed that the loss of activator function coincides with selective removal of the C-terminal domain (CTD)-hyperphosphorylated RNAP IIO along with NC2. Coimmunoprecipitation experiments with purified factors confirmed that NC2 interacts with RNAP IIO, but not with the unphosphorylated or hypophosphorylated RNAP IIA or CTD-less RNAP IIB forms. Finally, we demonstrate that, in contrast to previously published observations in cell-free systems reconstituted with purified factors, only the CTD-phosphorylated form of RNAP II can mediate activator function in the context of unfractionated HeLa nuclear extracts. These findings reveal an unexpected link between NC2 and transcription activation and suggest that regulation of RNAP II transcription through reversible CTD phosphorylation might be more complex than previously proposed.
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