ArticleProceedings of the National Academy of Sciences of the United States of America2000
Minimal exclusion of plasma membrane proteins during retroviral envelope formation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 41 citations in OpenAlex.
- Basic motifs target PSGL-1, CD43, and CD44 to plasma membrane sites where HIV-1 assembles.Journal of virology · 2015Article
- Mutating conserved cysteines in the alphavirus e2 glycoprotein causes virus-specific assembly defects.Journal of virology · 2012Article
- Retroviral env glycoprotein trafficking and incorporation into virions.Molecular biology international · 2012Article
- Mechanisms for Env glycoprotein acquisition by retroviruses.AIDS research and human retroviruses · 2011Review
- Virus assembly, allostery and antivirals.Trends in microbiology · 2011Review
- HIV-1 assembly differentially alters dynamics and partitioning of tetraspanins and raft components.Traffic (Copenhagen, Denmark) · 2010Article
- Host-encoded reporters for the detection and purification of multiple enveloped viruses.Journal of virological methods · 2010Article
- Purification of infectious human herpesvirus 6A virions and association of host cell proteins.Virology journal · 2007Article
- The fusion-controlling disulfide bond isomerase in retrovirus Env is triggered by protein destabilization.Journal of virology · 2005Article
- Passive and active inclusion of host proteins in human immunodeficiency virus type 1 gag particles during budding at the plasma membrane.Journal of virology · 2004Article
- Optimized lentiviral vector production and purification procedure prevents immune response after transduction of mouse brain.Gene therapy · 2003Article
- The Trojan exosome hypothesis.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Human immunodeficiency virus type 1 assembly and lipid rafts: Pr55(gag) associates with membrane domains that are largely resistant to Brij98 but sensitive to Triton X-100.Journal of virology · 2003Article
- Acid-induced movements in the glycoprotein shell of an alphavirus turn the spikes into membrane fusion mode.The EMBO journal · 2002Article
- Molecular organization of Mason-Pfizer monkey virus capsids assembled from Gag polyprotein in Escherichia coli.Journal of virology · 2002Article
- N-terminal cleavage fragment of glycosylated Gag is incorporated into murine oncornavirus particles.Journal of virology · 2001Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The retrovirus forms its envelope by budding at the plasma membrane (PM). This process is primarily driven by its cytoplasmic core-precursor protein, Gag, as shown by the efficient formation of virus-like Gag particles in the absence of its envelope protein, Env. Most interestingly, several studies have demonstrated incorporation of various PM proteins into retrovirus, but the underlying mechanism of this phenomenon has remained elusive. We have purified Moloney murine leukemia virus Gag particles by sedimentation in an iodixanol gradient and donor PMs by flotation in a sucrose gradient and compared their protein compositions at equal lipid basis. We found that most PM proteins are present at similar density in both membranes. The inclusion of PM proteins was unaffected by incorporation of Env protein into the envelope of the Gag particles and whether these were produced at high or low level in the cells. These findings indicate that most PM proteins become incorporated into the retrovirus envelope without significant sorting. This feature of retrovirus assembly should be considered when studying retrovirus functions and developing retrovirus vectors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.