ArticleJournal of bacteriology2000
Autodisplay: functional display of active beta-lactamase on the surface of Escherichia coli by the AIDA-I autotransporter.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 113 citations in OpenAlex.
- Engineering of Surface Display Systems inBiotech (Basel (Switzerland)) · 2026Article
- Bacterial Membrane Vesicles: Biogenesis, Functions, and Emerging Biotechnological Applications.Microorganisms · 2026Review
- A modular biodevice assembly tool kit for combinatorial screening and benchmarking multiple cell surface display platforms in Escherichia coli.Journal of biological engineering · 2026Article
- Outer Membrane Vesicles as a Versatile Platform for Vaccine Development: Engineering Strategies, Applications and Challenges.Journal of extracellular vesicles · 2025Review
- Structural basis of Fusobacterium nucleatum adhesin Fap2 interaction with receptors on cancer and immune cells.Nature communications · 2025Article
- Constructing Escherichia coli co-display systems for biodegradation of polyethylene terephthalate.Bioresources and bioprocessing · 2023Article
- Phylogenetic Classification and Functional Review of Autotransporters.Frontiers in immunology · 2022Review
- FimH-based display of functional eukaryotic proteins on bacteria surfaces.Scientific reports · 2019Article
- The Electrosome: A Surface-Displayed Enzymatic Cascade in a Biofuel Cell's Anode and a High-Density Surface-Displayed Biocathodic Enzyme.Nanomaterials (Basel, Switzerland) · 2017Article
- Poly-protein G-expressing bacteria enhance the sensitivity of immunoassays.Scientific reports · 2017Article
- Surface display of Aggregatibacter actinomycetemcomitans autotransporter Aae and dispersin B hybrid act as antibiofilm agents.Molecular oral microbiology · 2016Article
- Autodisplay of Human Hyaluronidase Hyal-1 on Escherichia coli and Identification of Plant-Derived Enzyme Inhibitors.Molecules (Basel, Switzerland) · 2015Article
- Functional display of heterotetrameric human protein kinase CK2 on Escherichia coli: a novel tool for drug discovery.Microbial cell factories · 2015Article
- An engineered autotransporter-based surface expression vector enables efficient display of Affibody molecules on OmpT-negative E. coli as well as protease-mediated secretion in OmpT-positive strains.Microbial cell factories · 2014Article
- Expression of β-glucuronidase on the surface of bacteria enhances activation of glucuronide prodrugs.Cancer gene therapy · 2013Article
- Probing the applicability of autotransporter based surface display with the EstA autotransporter of Pseudomonas stutzeri A15.Microbial cell factories · 2012Article
- Cell surface display of a β-glucosidase employing the type V secretion system on ethanologenic Escherichia coli for the fermentation of cellobiose to ethanol.Journal of industrial microbiology & biotechnology · 2012Article
- Characterization of Esterase A, a Pseudomonas stutzeri A15 Autotransporter.Applied and environmental microbiology · 2012Article
- IcsA autotransporter passenger promotes increased fusion protein expression on the cell surface.Microbial cell factories · 2012Article
- Surface display of Aeromonas hydrophila GAPDH in attenuated Vibrio anguillarum to develop a Noval multivalent vector vaccine.Marine biotechnology (New York, N.Y.) · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the protein family of immunoglobulin A1 protease-like autotransporters comprise multidomain precursors consisting of a C-terminal autotransporter domain that promotes the translocation of N-terminally attached passenger domains across the cell envelopes of gram-negative bacteria. Several autotransporter domains have recently been shown to efficiently promote the export of heterologous passenger domains, opening up an effective tool for surface display of heterologous proteins. Here we report on the autotransporter domain of the Escherichia coli adhesin involved in diffuse adherence (AIDA-I), which was genetically fused to the C terminus of the periplasmic enzyme beta-lactamase, leading to efficient expression of the fusion protein in E. coli. The beta-lactamase moiety of the fusion protein was presented on the bacterial surface in a stable manner, and the surface-located beta-lactamase was shown to be enzymatically active. Enzymatic activity was completely removed by protease treatment, indicating that surface display of beta-lactamase was almost quantitative. The periplasmic domain of the outer membrane protein OmpA was not affected by externally added proteases, demonstrating that the outer membranes of E. coli cells expressing the beta-lactamase AIDA-I fusion protein remained physiologically intact.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.