ArticleMolecular biology of the cell2000
Assembly and functional organization of the nucleolus: ultrastructural analysis of Saccharomyces cerevisiae mutants.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 68 citations in OpenAlex.
- Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions.Scientific reports · 2025Article
- In situ cryo-electron tomography reveals gradient organization of ribosome biogenesis in intact nucleoli.Nature communications · 2021Article
- Ribosomal proteins and human diseases: molecular mechanisms and targeted therapy.Signal transduction and targeted therapy · 2021Review
- The rDNA is biomolecular condensate formed by polymer-polymer phase separation and is sequestered in the nucleolus by transcription and R-loops.Nucleic acids research · 2021Article
- Visualization of Chromatin in the Yeast Nucleus and Nucleolus Using Hyperosmotic Shock.International journal of molecular sciences · 2021Article
- Review
- Review
- The C-terminal region of Net1 is an activator of RNA polymerase I transcription with conserved features from yeast to human.PLoS genetics · 2019Article
- Focal accumulation of preribosomes outside the nucleolus during metaphase-anaphase in budding yeast.RNA (New York, N.Y.) · 2017Article
- Article
- High resolution microscopy reveals the nuclear shape of budding yeast during cell cycle and in various biological states.Journal of cell science · 2016Article
- Decoding the principles underlying the frequency of association with nucleoli for RNA polymerase III-transcribed genes in budding yeast.Molecular biology of the cell · 2016Article
- Perturbations at the ribosomal genes loci are at the centre of cellular dysfunction and human disease.Cell & bioscience · 2014Review
- Structure and function in the budding yeast nucleus.Genetics · 2012Review
- Regulation of ribosomal RNA production by RNA polymerase I: does elongation come first?Genetics research international · 2012Article
- RNA polymerase I-specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycle.The Journal of cell biology · 2011Article
- Transcription-dependent rearrangements of actin and nuclear myosin I in the nucleolus.Histochemistry and cell biology · 2010Article
- Analysis of ribosome biogenesis factor-modules in yeast cells depleted from pre-ribosomes.Nucleic acids research · 2010Article
- Nucleolar separation from chromosomes during Aspergillus nidulans mitosis can occur without spindle forces.Molecular biology of the cell · 2009Article
- Inactivation of cleavage factor I components Rna14p and Rna15p induces sequestration of small nucleolar ribonucleoproteins at discrete sites in the nucleus.Molecular biology of the cell · 2008Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using Saccharomyces cerevisiae strains with genetically modified nucleoli, we show here that changing parameters as critical as the tandem organization of the ribosomal genes and the polymerase transcribing rDNA, although profoundly modifying the position and the shape of the nucleolus, only partially alter its functional subcompartmentation. High-resolution morphology achieved by cryofixation, together with ultrastructural localization of nucleolar proteins and rRNA, reveals that the nucleolar structure, arising upon transcription of rDNA from plasmids by RNA polymerase I, is still divided in functional subcompartments like the wild-type nucleolus. rRNA maturation is restricted to a fibrillar component, reminiscent of the dense fibrillar component in wild-type cells; a granular component is also present, whereas no fibrillar center can be distinguished, which directly links this latter substructure to rDNA chromosomal organization. Although morphologically different, the mininucleoli observed in cells transcribing rDNA with RNA polymerase II also contain a fibrillar subregion of analogous function, in addition to a dense core of unknown nature. Upon repression of rDNA transcription in this strain or in an RNA polymerase I thermosensitive mutant, the nucleolar structure falls apart (in a reversible manner), and nucleolar constituents partially relocate to the nucleoplasm, indicating that rRNA is a primary determinant for the assembly of the nucleolus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.