ArticleMolecular biology of the cell2000
Release of kinesin from vesicles by hsc70 and regulation of fast axonal transport.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 94 citations in OpenAlex.
- In vitro reconstitution reveals phosphoinositides as cargo-release factors and activators of the ARF6 GAP ADAP1.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Engagement of Neurotropic Viruses in Fast Axonal Transport: Mechanisms, Potential Role of Host Kinases and Implications for Neuronal Dysfunction.Frontiers in cellular neuroscience · 2021Article
- Tau and Axonal Transport Misregulation in Tauopathies.Advances in experimental medicine and biology · 2019Review
- Regulation of motor proteins, axonal transport deficits and adult-onset neurodegenerative diseases.Neurobiology of disease · 2017Review
- Hsc70 chaperone activity is required for the cytosolic slow axonal transport of synapsin.The Journal of cell biology · 2017Article
- A novel heat shock protein alpha 8 (Hspa8) molecular network mediating responses to stress- and ethanol-related behaviors.Neurogenetics · 2016Article
- Biochemical analysis of axon-specific phosphorylation events using isolated squid axoplasms.Methods in cell biology · 2016Article
- Organ Specific Proteomic Dissection of Selaginella bryopteris Undergoing Dehydration and Rehydration.Frontiers in plant science · 2016Article
- Isoform-selective Genetic Inhibition of Constitutive Cytosolic Hsp70 Activity Promotes Client Tau Degradation Using an Altered Co-chaperone Complement.The Journal of biological chemistry · 2015Article
- Functional Analysis of KIF3A and KIF3B during Spermiogenesis of Chinese Mitten Crab Eriocheir sinensis.PloS one · 2014Article
- Considerations About Risk Factors for Peripheral Neuropathies in Romanian HIV-Infected Patients.Current health sciences journal · 2014Article
- PtdIns(4)P regulates retromer-motor interaction to facilitate dynein-cargo dissociation at the trans-Golgi network.Nature cell biology · 2013Article
- Recycling of kinesin-1 motors by diffusion after transport.PloS one · 2013Article
- Inhibition of fast axonal transport by pathogenic SOD1 involves activation of p38 MAP kinase.PloS one · 2013Article
- Implication of Bemisia tabaci heat shock protein 70 in Begomovirus-whitefly interactions.Journal of virology · 2012Article
- Cytoplasmic trafficking, endosomal escape, and perinuclear accumulation of adeno-associated virus type 2 particles are facilitated by microtubule network.Journal of virology · 2012Article
- Axonal transport of APP and the spatial regulation of APP cleavage and function in neuronal cells.Experimental brain research · 2012Review
- Molecular composition of staufen2-containing ribonucleoproteins in embryonic rat brain.PloS one · 2010Article
- Kinesin-1/Hsc70-dependent mechanism of slow axonal transport and its relation to fast axonal transport.The EMBO journal · 2010Article
- Role of kinesin light chain-2 of kinesin-1 in the traffic of Na,K-ATPase-containing vesicles in alveolar epithelial cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The nature of kinesin interactions with membrane-bound organelles and mechanisms for regulation of kinesin-based motility have both been surprisingly difficult to define. Most kinesin is recovered in supernatants with standard protocols for purification of motor proteins, but kinesin recovered on membrane-bound organelles is tightly bound. Partitioning of kinesin between vesicle and cytosolic fractions is highly sensitive to buffer composition. Addition of either N-ethylmaleimide or EDTA to homogenization buffers significantly increased the fraction of kinesin bound to organelles. Given that an antibody against kinesin light chain tandem repeats also releases kinesin from vesicles, these observations indicated that specific cytoplasmic factors may regulate kinesin release from membranes. Kinesin light tandem repeats contain DnaJ-like motifs, so the effects of hsp70 chaperones were evaluated. Hsc70 released kinesin from vesicles in an MgATP-dependent and N-ethylmaleimide-sensitive manner. Recombinant kinesin light chains inhibited kinesin release by hsc70 and stimulated the hsc70 ATPase. Hsc70 actions may provide a mechanism to regulate kinesin function by releasing kinesin from cargo in specific subcellular domains, thereby effecting delivery of axonally transported materials.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.