ArticleMolecular biology of the cell2000
Spatially regulated recruitment of clathrin to the plasma membrane during capping and cell translocation.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- GxcM-Fbp17/RacC-WASP signaling regulates polarized cortex assembly in migrating cells via Arp2/3.The Journal of cell biology · 2023Article
- The Amoebal Model for Macropinocytosis.Sub-cellular biochemistry · 2022Article
- TheCells · 2021Review
- WASP Restricts Active Rac to Maintain Cells' Front-Rear Polarization.Current biology : CB · 2019Article
- WASP family proteins and formins compete in pseudopod- and bleb-based migration.The Journal of cell biology · 2018Article
- Turnover and flow of the cell membrane for cell migration.Scientific reports · 2017Article
- Fluorescent proteins: a cell biologist's user guide.Trends in cell biology · 2009Article
- Collective cell migration requires vesicular trafficking for chemoattractant delivery at the trailing edge.The Journal of cell biology · 2008Article
- The monomeric clathrin assembly protein, AP180, regulates contractile vacuole size in Dictyostelium discoideum.Molecular biology of the cell · 2006Article
- Monitoring clathrin-mediated endocytosis during synaptic activity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Morphology and dynamics of clathrin/GGA1-coated carriers budding from the trans-Golgi network.Molecular biology of the cell · 2003Article
- Caveolae are highly immobile plasma membrane microdomains, which are not involved in constitutive endocytic trafficking.Molecular biology of the cell · 2002Article
- A myosin I is involved in membrane recycling from early endosomes.The Journal of cell biology · 2000Article
- Ricin and viscumin bind to different sites of the cell membrane.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sectionsArticle
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Authors and funding
2 authors.
Funding
Abstract
Clathrin-coated vesicles bud from selected cellular membranes to traffic-specific intracellular proteins. To study the dynamic properties of clathrin-coated membranes, we expressed clathrin heavy chain tagged with green fluorescent protein (GFP) in Dictyostelium cells. GFP-clathrin was functional and retained the native properties of clathrin: the chimeric protein formed classic clathrin lattices on cellular membranes and also rescued phenotypic defects of clathrin null cells. GFP-clathrin distributed into punctate loci found throughout the cytoplasm, on the plasma membrane, and concentrated to a perinuclear location. These clathrin-coated structures were remarkably motile and capable of rapid and bidirectional transport across the cell. We identified two local domains of the plasma membrane as sites for clathrin recruitment in motile cells. First, as cells translocated or changed shape and retracted their tails, clathrin was transiently concentrated on the membrane at the back of the cell tail. Second, as cells capped their cell surface receptors, clathrin was recruited locally to the membrane under the tight cap of cross-linked receptors. This suggests that local sites for clathrin polymerization on specific domains of the plasma membrane undergo rapid and dynamic regulation in motile cells.
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