ArticleMolecular and cellular biology2000
RanGTP-binding protein NXT1 facilitates nuclear export of different classes of RNA in vitro.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
- The life and times of a tRNA.RNA (New York, N.Y.) · 2023Review
- Nucleoporin TPR promotes tRNA nuclear export and protein synthesis in lung cancer cells.PLoS genetics · 2021Article
- Specialization of theGenes & development · 2019Article
- The coming-of-age of nucleocytoplasmic transport in motor neuron disease and neurodegeneration.Cellular and molecular life sciences : CMLS · 2019Review
- tRNA Processing and Subcellular Trafficking Proteins Multitask in Pathways for Other RNAs.Frontiers in genetics · 2019Review
- Intron retention in viruses and cellular genes: Detention, border controls and passports.Wiley interdisciplinary reviews. RNA · 2018Review
- tRNA dynamics between the nucleus, cytoplasm and mitochondrial surface: Location, location, location.Biochimica et biophysica acta. Gene regulatory mechanisms · 2018Review
- mRNA export in the apicomplexan parasite Toxoplasma gondii: emerging divergent components of a crucial pathway.Parasites & vectors · 2018Review
- Sharing the load: Mex67-Mtr2 cofunctions with Los1 in primary tRNA nuclear export.Genes & development · 2017Article
- NXT1, a Novel Influenza A NP Binding Protein, Promotes the Nuclear Export of NP via a CRM1-Dependent Pathway.Viruses · 2016Article
- RNA Export through the NPC in Eukaryotes.Genes · 2015Review
- Human regulator of telomere elongation helicase 1 (RTEL1) is required for the nuclear and cytoplasmic trafficking of pre-U2 RNA.Nucleic acids research · 2015Article
- Mutations in tap uncouple RNA export activity from translocation through the nuclear pore complex.Molecular biology of the cell · 2006Article
- In vivo BiFC analysis of Y14 and NXF1 mRNA export complexes: preferential localization within and around SC35 domains.The Journal of cell biology · 2006Article
- Optical microwell assay of membrane transport kinetics.Biophysical journal · 2003Article
- In vitro analysis of nuclear mRNA export using molecular beacons for target detection.Nucleic acids research · 2003Article
- Nuclear export of mRNA by TAP/NXF1 requires two nucleoporin-binding sites but not p15.Molecular and cellular biology · 2002Article
- Transport into and out of the nucleus.Microbiology and molecular biology reviews : MMBR · 2001Review
- NXF1/p15 heterodimers are essential for mRNA nuclear export in Drosophila.RNA (New York, N.Y.) · 2001Article
- NXT1 (p15) is a crucial cellular cofactor in TAP-dependent export of intron-containing RNA in mammalian cells.Molecular and cellular biology · 2001Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To better characterize the mechanisms responsible for RNA export from the nucleus, we developed an in vitro assay based on the use of permeabilized HeLa cells. This new assay supports nuclear export of U1 snRNA, tRNA, and mRNA in an energy- and Xenopus extract-dependent manner. U1 snRNA export requires a 5' monomethylated cap structure, the nuclear export signal receptor CRM1, and the small GTPase Ran. In contrast, mRNA export does not require the participation of CRM1. We show here that NXT1, an NTF2-related protein that binds directly to RanGTP, strongly stimulates export of U1 snRNA, tRNA, and mRNA. The ability of NXT1 to promote export is dependent on its capacity to bind RanGTP. These results support the emerging view that NXT1 is a general export factor, functioning on both CRM1-dependent and CRM1-independent pathways of RNA export.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.