ArticleJournal of virology2000
Analysis of cellular factors that mediate nuclear export of RNAs bearing the Mason-Pfizer monkey virus constitutive transport element.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Virus Infection and mRNA Nuclear Export.International journal of molecular sciences · 2023Review
- Multiple nuclear-replicating viruses require the stress-induced protein ZC3H11A for efficient growth.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Leukemia-Associated Nup214 Fusion Proteins Disturb the XPO1-Mediated Nuclear-Cytoplasmic Transport Pathway and Thereby the NF-κB Signaling Pathway.Molecular and cellular biology · 2016Article
- Localization of nucleoporin Tpr to the nuclear pore complex is essential for Tpr mediated regulation of the export of unspliced RNA.PloS one · 2012Article
- Role of the RNA recognition motif of the E1B 55 kDa protein in the adenovirus type 5 infectious cycle.Virology · 2011Article
- Foamy virus nuclear RNA export is distinct from that of other retroviruses.Journal of virology · 2011Article
- Export of adenoviral late mRNA from the nucleus requires the Nxf1/Tap export receptor.Journal of virology · 2011Article
- Analysis of synergy between divergent simple retrovirus posttranscriptional control elements.Virology · 2003Article
- Karyopherin beta 2B participates in mRNA export from the nucleus.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Nuclear export of mRNA by TAP/NXF1 requires two nucleoporin-binding sites but not p15.Molecular and cellular biology · 2002Article
- CRM1-dependent function of a cis-acting RNA export element.Molecular and cellular biology · 2002Article
- An element in the 3' untranslated region of human LINE-1 retrotransposon mRNA binds NXF1(TAP) and can function as a nuclear export element.RNA (New York, N.Y.) · 2002Article
- The crystal structure and mutational analysis of a novel RNA-binding domain found in the human Tap nuclear mRNA export factor.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Formation of Tap/NXT1 heterodimers activates Tap-dependent nuclear mRNA export by enhancing recruitment to nuclear pore complexes.Molecular and cellular biology · 2002Article
- NXF1/p15 heterodimers are essential for mRNA nuclear export in Drosophila.RNA (New York, N.Y.) · 2001Article
- Using viral species specificity to define a critical protein/RNA interaction surface.Genes & development · 2001Article
- NXT1 (p15) is a crucial cellular cofactor in TAP-dependent export of intron-containing RNA in mammalian cells.Molecular and cellular biology · 2001Article
- NXT1 is necessary for the terminal step of Crm1-mediated nuclear export.The Journal of cell biology · 2001Article
- TAP (NXF1) belongs to a multigene family of putative RNA export factors with a conserved modular architecture.Molecular and cellular biology · 2000Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is now convincing evidence that the human Tap protein plays a critical role in mediating the nuclear export of mRNAs that contain the Mason-Pfizer monkey virus constitutive transport element (CTE) and significant evidence that Tap also participates in global poly(A)(+) RNA export. Previously, we had mapped carboxy-terminal sequences in Tap that serve as an essential nucleocytoplasmic shuttling domain, while others had defined an overlapping Tap sequence that can bind to the FG repeat domains of certain nucleoporins. Here, we demonstrate that these two biological activities are functionally correlated. Specifically, mutations in Tap that block nucleoporin binding also block both nucleocytoplasmic shuttling and the Tap-dependent nuclear export of CTE-containing RNAs. In contrast, mutations that do not inhibit nucleoporin binding also fail to affect Tap shuttling. Together, these data indicate that Tap belongs to a novel class of RNA export factors that can target bound RNA molecules directly to the nuclear pore without the assistance of an importin beta-like cofactor. In addition to nucleoporins, Tap has also been proposed to interact with a cellular cofactor termed p15. Although we were able to confirm that Tap can indeed bind p15 specifically both in vivo and in vitro, a mutation in Tap that blocked p15 binding only modestly inhibited CTE-dependent nuclear RNA export. However, p15 did significantly enhance the affinity of Tap for the CTE in vitro and readily formed a ternary complex with Tap on the CTE. This result suggests that p15 may play a significant role in the recruitment of the Tap nuclear export factor to target RNA molecules in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.