ArticleMolecular microbiology2000
PAS domain residues involved in signal transduction by the Aer redox sensor of Escherichia coli.
Article in Molecular microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 117 citations in OpenAlex.
- Signaling mechanism of the transmembrane energy receptor Aer.bioRxiv : the preprint server for biology · 2026Article
- Aer is a bidirectional redox sensor mediating negative chemotaxis to antibiotic-induced ROS inmBio · 2026Article
- A Two-Protein Chemoreceptor Complex Regulates Oxygen Thresholds in Bacterial Magneto-Aerotaxis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- ThemBio · 2025Article
- Characterization ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Two-Component System Sensor Kinases from Asgardian Archaea May Be Witnesses to Eukaryotic Cell Evolution.Molecules (Basel, Switzerland) · 2023Article
- Leptospira interrogans Aer2: an Unusual Membrane-Bound PAS-Heme Oxygen Sensor.Journal of bacteriology · 2022Article
- The aryl hydrocarbon receptor as a model PAS sensor.Toxicology reports · 2022Review
- Effects of organic acids on the chemotaxis profiles and biocontrol traits of antagonistic bacterial endophytes against root-rot disease in Panax notoginseng.Antonie van Leeuwenhoek · 2021Article
- A PilZ-Containing Chemotaxis Receptor Mediates Oxygen and Wheat Root Sensing inFrontiers in microbiology · 2019Article
- In silico characterization of a novel putative aerotaxis chemosensory system in the myxobacterium, Corallococcus coralloides.BMC genomics · 2018Article
- Combining protein sequence, structure, and dynamics: A novel approach for functional evolution analysis of PAS domain superfamily.Protein science : a publication of the Protein Society · 2018Article
- Bacterial Energy Sensor Aer Modulates the Activity of the Chemotaxis Kinase CheA Based on the Redox State of the Flavin Cofactor.The Journal of biological chemistry · 2016Article
- The Aerotactic Response of Caulobacter crescentus.Biophysical journal · 2016Article
- Delineating PAS-HAMP interaction surfaces and signalling-associated changes in the aerotaxis receptor Aer.Molecular microbiology · 2016Article
- Bacterial chemoreceptors and chemoeffectors.Cellular and molecular life sciences : CMLS · 2015Review
- Identification and characterization of three Vibrio alginolyticus non-coding RNAs involved in adhesion, chemotaxis, and motility processes.Frontiers in cellular and infection microbiology · 2015Article
- Plasmid-encoded MCP is involved in virulence, motility, and biofilm formation of Cronobacter sakazakii ATCC 29544.Infection and immunity · 2015Article
- The cytoplasmic PASC domain of the sensor kinase DcuS of Escherichia coli: role in signal transduction, dimer formation, and DctA interaction.MicrobiologyOpen · 2013Article
- Charting the signal trajectory in a light-oxygen-voltage photoreceptor by random mutagenesis and covariance analysis.The Journal of biological chemistry · 2013Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
PAS domains sense oxygen, redox potential and light, and are implicated in behaviour, circadian rhythmicity, development and metabolic regulation. Although PAS domains are widespread in archaea, bacteria and eukaryota, the mechanism of signal transduction has been elucidated only for the bacterial photo sensor PYP and oxygen sensor FixL. We investigated the signalling mechanism in the PAS domain of Aer, the redox potential sensor and aerotaxis transducer in Escherichia coli. Forty-two residues in Aer were substituted using cysteine-replacement mutagenesis. Eight mutations resulted in a null phenotype for aerotaxis, the behavioural response to oxygen. Four of them also led to the loss of the non-covalently bound FAD cofactor. Three mutant Aer proteins, N34C, F66C and N85C, transmitted a constant signal-on bias. One mutation, Y111C, inverted signalling by the transducer so that positive stimuli produced negative signals and vice versa. Residues critical for signalling were mapped onto a three-dimensional model of the Aer PAS domain, and an FAD-binding site and 'active site' for signal transduction are proposed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.