ArticleProceedings of the National Academy of Sciences of the United States of America2000
Adaptive variation in lactate dehydrogenase-B gene expression: role of a stress-responsive regulatory element.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 107 citations in OpenAlex.
- Steep, coincident, and concordant clines in mitochondrial and nuclear-encoded genes in a hybrid zone between subspecies of Atlantic killifish, Fundulus heteroclitus.Ecology and evolution · 2016Article
- A Novel Candidate Gene for Temperature-Dependent Sex Determination in the Common Snapping Turtle.Genetics · 2016Article
- Towards the identification of the loci of adaptive evolution.Methods in ecology and evolution · 2015Review
- The Elizabeth River Story: A Case Study in Evolutionary Toxicology.Journal of toxicology and environmental health. Part B, Critical reviews · 2015Review
- Article
- Gene expression variation in duplicate lactate dehydrogenase genes: do ecological species show distinct responses?PloS one · 2014Article
- Oceanographic connectivity and environmental correlates of genetic structuring in Atlantic herring in the Baltic Sea.Evolutionary applications · 2013Article
- Article
- Role of DNA methylation of AHR1 and AHR2 promoters in differential sensitivity to PCBs in Atlantic Killifish, Fundulus heteroclitus.Aquatic toxicology (Amsterdam, Netherlands) · 2011Article
- Seasonal changes in hepatic gene expression reveal modulation of multiple processes in rainbow smelt (Osmerus mordax).Marine biotechnology (New York, N.Y.) · 2010Article
- AHR2 mediates cardiac teratogenesis of polycyclic aromatic hydrocarbons and PCB-126 in Atlantic killifish (Fundulus heteroclitus).Aquatic toxicology (Amsterdam, Netherlands) · 2010Article
- Genomic approaches with natural fish populations.Journal of fish biology · 2010Review
- Genomic signatures of local directional selection in a high gene flow marine organism; the Atlantic cod (Gadus morhua).BMC evolutionary biology · 2009Article
- Comparative characterization of a temperature responsive gene (lactate dehydrogenase-B, ldh-b) in two congeneric tropical fish, Lates calcarifer and Lates niloticus.International journal of biological sciences · 2009Article
- Evidence of spatially varying selection acting on four chromatin-remodeling loci in Drosophila melanogaster.Genetics · 2008Article
- Thermal limits and adaptation in marine Antarctic ectotherms: an integrative view.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2007Article
- Fundulus as the premier teleost model in environmental biology: opportunities for new insights using genomics.Comparative biochemistry and physiology. Part D, Genomics & proteomics · 2007Article
- Recombinational micro-evolution of functionally different metallothionein promoter alleles from Orchesella cincta.BMC evolutionary biology · 2007Article
- Tissue-specific activity of the blind mole rat and the two nucleotide-mutated mouse alphaB-crystallin promoter in transgenic mice.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- High-diversity genes in the Arabidopsis genome.Genetics · 2005Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although changes in gene regulation may play an important role in adaptive evolution, there have been few attempts to investigate the molecular mechanisms responsible for adaptively significant variation in gene expression. Here we describe the mechanism underlying an adaptive difference in the expression of the lactate dehydrogenase-B gene (Ldh-B) between northern and southern populations of the fish Fundulus heteroclitus. Ldh-B regulatory sequences from northern and southern individuals, coupled to a luciferase reporter gene, were introduced into the livers of live fish. Deletion studies indicated that sequence changes between 400 and 500 bp upstream of the transcription start site resulted in a 2-fold difference in reporter gene transcription. These sequence changes can account for the previously observed 2-fold difference in Ldh-B transcription between populations. Variation in transcription factors did not play an important role. Sequences within the functionally important region resemble a mammary tumor virus glucocorticoid responsive element (MTV-GRE) in southern alleles, whereas northern alleles differ from the consensus by 1 bp. To test the hypothesis that this element is involved in the variation between populations of F. heteroclitus, we exposed transiently transgenic fish containing Ldh-B regulatory sequence/reporter gene constructs to handling stress or injected cortisol. Both treatments increased reporter gene transcription driven by southern alleles but not northern alleles, as expected if an MTV-GRE sequence were involved. This finding suggests that sequence variation in a GRE is the cause of the adaptive differences in Ldh-B gene expression between populations and demonstrates that small changes in gene regulatory sequences can have important evolutionary consequences.
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