ArticleProceedings of the National Academy of Sciences of the United States of America2000
Rational design of nascent metalloenzymes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 113 citations in OpenAlex.
- Strand-Swapped SH3 Domain Dimer with Superoxide Dismutase Activity.ACS central science · 2025Article
- Rational De Novo Design of a Cu Metalloenzyme for Superoxide Dismutation.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Minimal Heterochiral de Novo Designed 4Fe-4S Binding Peptide Capable of Robust Electron Transfer.Journal of the American Chemical Society · 2018Article
- Computational Redesign of Thioredoxin Is Hypersensitive toward Minor Conformational Changes in the Backbone Template.Journal of molecular biology · 2016Article
- Use of an Improved Matching Algorithm to Select Scaffolds for Enzyme Design Based on a Complex Active Site Model.PloS one · 2016Article
- Metal Stabilization of Collagen and de Novo Designed Mimetic Peptides.Biochemistry · 2015Article
- Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently.Chemical Society reviews · 2015Review
- Protein design: toward functional metalloenzymes.Chemical reviews · 2014Review
- Designing hydrolytic zinc metalloenzymes.Biochemistry · 2014Review
- Designing functional metalloproteins: from structural to catalytic metal sites.Coordination chemistry reviews · 2013Article
- Systematic optimization model and algorithm for binding sequence selection in computational enzyme design.Protein science : a publication of the Protein Society · 2013Article
- Influence of active site location on catalytic activity in de novo-designed zinc metalloenzymes.Journal of the American Chemical Society · 2013Article
- Engineered proteins: redox properties and their applications.Antioxidants & redox signaling · 2012Review
- Azurin as a protein scaffold for a low-coordinate nonheme iron site with a small-molecule binding pocket.Journal of the American Chemical Society · 2012Article
- Apoprotein isolation and activation, and vibrational structure of the Helicobacter mustelae iron urease.Journal of inorganic biochemistry · 2012Article
- Catalysis by a de novo zinc-mediated protein interface: implications for natural enzyme evolution and rational enzyme engineering.Biochemistry · 2012Article
- Computational approaches for rational design of proteins with novel functionalities.Computational and structural biotechnology journal · 2012Review
- Binding pocket optimization by computational protein design.PloS one · 2012Article
- A matching algorithm for catalytic residue site selection in computational enzyme design.Protein science : a publication of the Protein Society · 2011Article
- Designing artificial enzymes by intuition and computation.Nature chemistry · 2010Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Understanding the early genesis of new enzymatic functions is one of the challenges in protein design, mechanistic enzymology, and molecular evolution. We have experimentally mimicked starting points in this process by introducing primitive iron and oxygen binding sites at various locations in thioredoxin, a small protein lacking metal centers, by using computational design. These rudimentary active sites show emerging enzymatic activities that select to varying degrees between different oxygen chemistries. Even within these nascent enzymes, mechanisms by which different reactions are controlled can be discerned. These involve both stabilizing and destabilizing interactions imposed on the metal center by the surrounding protein matrix.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.