ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2000
Generation of transgenic mice and germline transmission of a mammalian artificial chromosome introduced into embryos by pronuclear microinjection.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 86 citations in OpenAlex.
- Prospects of Non-Coding Elements in Genomic DNA Based Gene Therapy.Current gene therapy · 2022Review
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- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Combinations of chromosome transfer and genome editing for the development of cell/animal models of human disease and humanized animal models.Journal of human genetics · 2018Review
- Successful transmission and transcriptional deployment of a human chromosome via mouse male meiosis.eLife · 2016Article
- De novo formed satellite DNA-based mammalian artificial chromosomes and their possible applications.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Human artificial chromosomes for future biomedicine.Cell cycle (Georgetown, Tex.) · 2015Article
- Transcription of a protein-coding gene on B chromosomes of the Siberian roe deer (Capreolus pygargus).BMC biology · 2013Article
- Meiotic transmission of an in vitro-assembled autonomous maize minichromosome.PLoS genetics · 2007Article
- Flow analysis and sorting of microchromosomes (<3 Mb).Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2007Article
- Artificial and engineered chromosomes: developments and prospects for gene therapy.Chromosoma · 2005Review
- Differential rates of local and global homogenization in centromere satellites from Arabidopsis relatives.Genetics · 2005Article
- Polymers for DNA delivery.Molecules (Basel, Switzerland) · 2005Review
- A mammalian artificial chromosome engineering system (ACE System) applicable to biopharmaceutical protein production, transgenesis and gene-based cell therapy.Nucleic acids research · 2004Article
- Gene Therapy: The Potential Applicability of Gene Transfer Technology to the Human Germline.International journal of medical sciences · 2004Article
- Chromosome engineering: prospects for gene therapy.Gene therapy · 2002Article
- Retrofitting of a satellite repeat DNA-based murine artificial chromosome (ACes) to contain loxP recombination sites.Gene therapy · 2002Article
- Biology's last taboo.Nature · 2001Article
- Construction of neocentromere-based human minichromosomes by telomere-associated chromosomal truncation.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Efficient in-vitro transfer of a 60-Mb mammalian artificial chromosome into murine and hamster cells using cationic lipids and dendrimers.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2001Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have generated transgenic mice by pronuclear microinjection of a murine satellite DNA-based artificial chromosome (SATAC). As 50% of the founder progeny were SATAC-positive, this demonstrates that SATAC transmission through the germline had occurred. FISH analyses of metaphase chromosomes from mitogen-activated peripheral blood lymphocytes from both the founder and progeny revealed that the SATAC was maintained as a discrete chromosome and that it had not integrated into an endogenous chromosome. To our knowledge, this is the first report of the germline transmission of a genetically engineered mammalian artificial chromosome within transgenic animals generated through pronuclear microinjection. We have also shown that murine SATACs can be similarly introduced into bovine embryos. The use of embryo microinjection to generate transgenic mammals carrying genetically engineered chromosomes provides a novel method by which the unique advantages of chromosome-based gene delivery systems can be exploited.
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