ArticleProceedings of the National Academy of Sciences of the United States of America2000
Stepping rotation of F1-ATPase visualized through angle-resolved single-fluorophore imaging.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed.
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- Artificial molecular machines: Design and observation.Smart molecules : open access · 2023Review
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- Mechanisms of hexameric helicases.Critical reviews in biochemistry and molecular biology · 2021Review
- The six steps of the complete FNature communications · 2021Article
- Measuring 3D orientation of nanocrystals via polarized luminescence of rare-earth dopants.Nature communications · 2021Article
- Semiconductor Nanocrystals for Biological Imaging and Fluorescence Spectroscopy.Advances in experimental medicine and biology · 2021Article
- Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy.Nature communications · 2019Article
- Structural Asymmetry and Kinetic Limping of Single Rotary F-ATP Synthases.Molecules (Basel, Switzerland) · 2019Review
- Use of Single Molecule Fluorescence Polarization Microscopy to Study Protein Conformation and Dynamics of Kinesin-Microtubule Complexes.Methods in molecular biology (Clifton, N.J.) · 2018Article
- The uniqueness of subunit α of mycobacterial F-ATP synthases: An evolutionary variant for niche adaptation.The Journal of biological chemistry · 2017Article
- DNA-Endonuclease Complex Dynamics by Simultaneous FRET and Fluorophore Intensity in Evanescent Field.Biophysical journal · 2017Article
- Single molecule optical measurements of orientation and rotations of biological macromolecules.Methods and applications in fluorescence · 2016Article
- Development of Potent Antiviral Drugs Inspired by Viral Hexameric DNA-Packaging Motors with Revolving Mechanism.Journal of virology · 2016Review
- Janus particles for biological imaging and sensing.The Analyst · 2016Review
- Single Molecule 3D Orientation in Time and Space: A 6D Dynamic Study on Fluorescently Labeled Lipid Membranes.Journal of fluorescence · 2016Article
- Discovery of a new method for potent drug development using power function of stoichiometry of homomeric biocomplexes or biological nanomotors.Expert opinion on drug delivery · 2016Article
- Artificial Molecular Machines.Chemical reviews · 2015Review
12 more citing papers are in PubMed but not listed here.
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7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Orientation dependence of single-fluorophore intensity was exploited in order to videotape conformational changes in a protein machine in real time. The fluorophore Cy3 attached to the central subunit of F(1)-ATPase revealed that the subunit rotates in the molecule in discrete 120 degrees steps and that each step is driven by the hydrolysis of one ATP molecule. These results, unlike those from the previous study under a frictional load, show that the 120 degrees stepping is a genuine property of this molecular motor. The data also show that the rate of ATP binding is insensitive to the load exerted on the rotor subunit.
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