ArticleGenetics2000
Loss of Rhb1, a Rheb-related GTPase in fission yeast, causes growth arrest with a terminal phenotype similar to that caused by nitrogen starvation.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 107 citations in OpenAlex.
- The Central FacilitaTOR: Coordinating Transcription and Translation in Eukaryotes.International journal of molecular sciences · 2025Review
- Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.PLoS biology · 2024Article
- Rapamycin-sensitive mechanisms confine the growth of fission yeast below the temperatures detrimental to cell physiology.iScience · 2024Article
- The fission yeast FLCN/FNIP complex augments TORC1 repression or activation in response to amino acid (AA) availability.iScience · 2021Article
- Review
- The TOR kinase pathway is relevant for nitrogen signaling and antagonism of the mycoparasite Trichoderma atroviride.PloS one · 2021Article
- Review
- Import of extracellular ATP in yeast and man modulates AMPK and TORC1 signalling.Journal of cell science · 2019Article
- Whi2 signals low leucine availability to halt yeast growth and cell death.FEMS yeast research · 2018Review
- The Target of Rapamycin and Mechanisms of Cell Growth.International journal of molecular sciences · 2018Review
- Article
- TORC1 and TORC2 converge to regulate the SAGA co-activator in response to nutrient availability.EMBO reports · 2017Article
- Amino acid and small GTPase regulation of mTORC1.Cellular logistics · 2017Review
- Review
- Nitrogen regulates AMPK to control TORC1 signaling.Current biology : CB · 2015Article
- Rhb1 regulates the expression of secreted aspartic protease 2 through the TOR signaling pathway in Candida albicans.Eukaryotic cell · 2012Article
- Regulation of TOR by small GTPases.EMBO reports · 2012Review
- Fission yeast TORC1 regulates phosphorylation of ribosomal S6 proteins in response to nutrients and its activity is inhibited by rapamycin.Journal of cell science · 2010Article
- Distinctive responses to nitrogen starvation in the dominant active mutants of the fission yeast Rheb GTPase.Genetics · 2009Article
- Early development of Moniliophthora perniciosa basidiomata and developmentally regulated genes.BMC microbiology · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Rheb GTPase is most similar in primary sequence to the Ras, Rap, R-Ras, and Ral GTPases, which regulate cell growth and differentiation in many cell types. A likely fission yeast homologue of mammalian Rheb, which we designated Rhb1, was identified by genome sequencing. Our investigation of rhb1 showed that rhb1(-) cells arrested cell growth and division with a terminal phenotype similar to that of nitrogen-starved cells. In particular, cells depleted of Rhb1 arrested as small, round cells with 1N DNA content, arrested more quickly in low-nitrogen medium, and induced expression of fnx1 and mei2 mRNA, two mRNAs that were normally induced by nitrogen starvation. Since mammalian Rheb binds and may regulate Raf-1, a Ras effector, we tested for functional overlap between Ras1 and Rhb1 in fission yeast. This analysis showed that Ras1 overexpression did not suppress rhb1(-) mutant phenotypes, Rhb1 overexpression did not suppress ras1(-) mutant phenotypes, and ras1(-) rhb1(-) double mutants had phenotypes equal to the sum of the corresponding single-mutant phenotypes. Hence, there is no evidence for overlapping functions between Ras1 and Rhb1. On the basis of this study, we hypothesize that Rhb1 negatively regulates entry into stationary phase when extracellular nitrogen levels are adequate for growth. If this hypothesis is correct, then Rhb1 and Ras1 regulate alternative responses to limiting nutrients.
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