ArticleThe EMBO journal2000
Selective instability of Orc1 protein accounts for the absence of functional origin recognition complexes during the M-G(1) transition in mammals.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 92 citations in OpenAlex.
- Starting DNA Synthesis: Initiation Processes during the Replication of Chromosomal DNA in Humans.Genes · 2024Review
- Distinct roles of Arabidopsis ORC1 proteins in DNA replication and heterochromatic H3K27me1 deposition.Nature communications · 2023Article
- A new class of disordered elements controls DNA replication through initiator self-assembly.eLife · 2019Article
- Mechanisms and regulation of DNA replication initiation in eukaryotes.Critical reviews in biochemistry and molecular biology · 2017Review
- Proteomics Analysis with a Nano Random Forest Approach Reveals Novel Functional Interactions Regulated by SMC Complexes on Mitotic Chromosomes.Molecular & cellular proteomics : MCP · 2016Article
- Orc1 Binding to Mitotic Chromosomes Precedes Spatial Patterning during G1 Phase and Assembly of the Origin Recognition Complex in Human Cells.The Journal of biological chemistry · 2015Article
- Regulating DNA replication in eukarya.Cold Spring Harbor perspectives in biology · 2013Review
- Epstein-Barr nuclear antigen 1 (EBNA1)-dependent recruitment of origin recognition complex (Orc) on oriP of Epstein-Barr virus with purified proteins: stimulation by Cdc6 through its direct interaction with EBNA1.The Journal of biological chemistry · 2012Article
- Mediators of reprogramming: transcription factors and transitions through mitosis.Nature reviews. Molecular cell biology · 2008Review
- Global regulation of genome duplication in eukaryotes: an overview from the epifluorescence microscope.Chromosoma · 2008Review
- Site-specific interaction of the murine pre-replicative complex with origin DNA: assembly and disassembly during cell cycle transit and differentiation.Nucleic acids research · 2007Article
- Binding of AlF-C, an Orc1-binding transcriptional regulator, enhances replicator activity of the rat aldolase B origin.Molecular and cellular biology · 2006Article
- The BAH domain facilitates the ability of human Orc1 protein to activate replication origins in vivo.The EMBO journal · 2006Article
- Identification of new human origins of DNA replication by an origin-trapping assay.Molecular and cellular biology · 2006Article
- Ubiquitylation, phosphorylation and Orc2 modulate the subcellular location of Orc1 and prevent it from inducing apoptosis.Journal of cell science · 2006Article
- Chinese hamster ORC subunits dynamically associate with chromatin throughout the cell-cycle.Experimental cell research · 2005Article
- Human Orc2 localizes to centrosomes, centromeres and heterochromatin during chromosome inheritance.The EMBO journal · 2004Article
- Role for Cdk1 (Cdc2)/cyclin A in preventing the mammalian origin recognition complex's largest subunit (Orc1) from binding to chromatin during mitosis.Molecular and cellular biology · 2004Article
- Dynamics of pre-replication complex proteins during the cell division cycle.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2004Review
- Degradation of origin recognition complex large subunit by the anaphase-promoting complex in Drosophila.The EMBO journal · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate the events leading to initiation of DNA replication in mammalian chromosomes, the time when hamster origin recognition complexes (ORCs) became functional was related to the time when Orc1, Orc2 and Mcm3 proteins became stably bound to hamster chromatin. Functional ORCs, defined as those able to initiate DNA replication, were absent during mitosis and early G(1) phase, and reappeared as cells progressed through G(1) phase. Immunoblotting analysis revealed that hamster Orc1 and Orc2 proteins were present in nuclei at equivalent concentrations throughout the cell cycle, but only Orc2 was stably bound to chromatin. Orc1 and Mcm3 were easily eluted from chromatin during mitosis and early G(1) phase, but became stably bound during mid-G(1) phase, concomitant with the appearance of a functional pre-replication complex at a hamster replication origin. Since hamster Orc proteins are closely related to their human and mouse homologs, the unexpected behavior of hamster Orc1 provides a novel mechanism in mammals for delaying assembly of pre-replication complexes until mitosis is complete and a nuclear structure has formed.
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