ArticleThe EMBO journal2000
Expression of the ORF-2 protein of the human respiratory syncytial virus M2 gene is initiated by a ribosomal termination-dependent reinitiation mechanism.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 76 citations in OpenAlex.
- A bicistronic viral genome uses a compact type IV IRES near its 3' end to express a transmembrane protein.Cell reports · 2026Article
- A conserved class of viral RNA structures regulates translation reinitiation through dynamic ribosome interactions.Cell reports · 2025Article
- Principles, mechanisms, and biological implications of translation termination-reinitiation.RNA (New York, N.Y.) · 2023Article
- Distinctive features of the respiratory syncytial virus priming loop compared to other non-segmented negative strand RNA viruses.PLoS pathogens · 2022Article
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- Deep splicing plasticity of the human adenovirus type 5 transcriptome drives virus evolution.Communications biology · 2020Article
- A unique insert in the genomes of high-risk human papillomaviruses with a predicted dual role in conferring oncogenic risk.F1000Research · 2019Article
- Please do not recycle! Translation reinitiation in microbes and higher eukaryotes.FEMS microbiology reviews · 2018Review
- Know Your Enemy: Successful Bioinformatic Approaches to Predict Functional RNA Structures in Viral RNAs.Frontiers in microbiology · 2017Review
- Review
- Cellular mRNAs access second ORFs using a novel amino acid sequence-dependent coupled translation termination-reinitiation mechanism.RNA (New York, N.Y.) · 2014Article
- Modulation of host adaptive immunity by hRSV proteins.Virulence · 2014Review
- Article
- APE-type non-LTR retrotransposons of multicellular organisms encode virus-like 2A oligopeptide sequences, which mediate translational recoding during protein synthesis.Molecular biology and evolution · 2013Article
- The comparative genomics of human respiratory syncytial virus subgroups A and B: genetic variability and molecular evolutionary dynamics.Journal of virology · 2013Article
- An upstream open reading frame modulates ebola virus polymerase translation and virus replication.PLoS pathogens · 2013Article
- Dual short upstream open reading frames control translation of a herpesviral polycistronic mRNA.PLoS pathogens · 2013Article
- Non-canonical translation in RNA viruses.The Journal of general virology · 2012Review
- Animal virus schemes for translation dominance.Current opinion in virology · 2011Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
Translation of the open reading frame 2 (ORF-2) of the human respiratory syncytial virus M2 gene initiates at one of the three initiation codons located upstream of the termination codon for the first ORF. Replacement of ORF-2 with the major ORF of the chloramphenicol acetyltransferase reporter gene followed by systematic mutagenesis of the putative initiation codons demonstrated the usage of these codons as the translational initiators for ORF-2 expression both in vitro and in vivo. While the efficiency of translation was maintained when only the first and second AUG codons were preserved in vivo, there was no apparent preference in vitro for any of the three codons when only one was present. Mutagenesis studies showed that the location of the termination codon of ORF-1 protein plays a crucial role in directing translation of ORF-2 from the upstream initiation codons in vivo. This indicates that the second ORF is accessed by the ribosomes that are departing from the first ORF and that these ribosomes reinitiate on AUG codons 5' to the point of translation termination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.