ArticleThe Journal of physiology2000
Effects of arachidonic acid on unitary calcium currents in rat sympathetic neurons.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- Roles of endogenous ether lipids and associated PUFAs in the regulation of ion channels and their relevance for disease.Journal of lipid research · 2020Review
- Modulation of CaBMC research notes · 2018Article
- Actions and Mechanisms of Polyunsaturated Fatty Acids on Voltage-Gated Ion Channels.Frontiers in physiology · 2017Review
- Characterization of ST14A Cells for Studying Modulation of Voltage-Gated Calcium Channels.PloS one · 2015Article
- Imaging large cohorts of single ion channels and their activity.Frontiers in endocrinology · 2013Review
- Acid-sensing ion channels (ASICs): therapeutic targets for neurological diseases and their regulation.BMB reports · 2013Review
- N-Arachidonyl glycine does not activate G protein-coupled receptor 18 signaling via canonical pathways.Molecular pharmacology · 2013Article
- (R)-roscovitine prolongs the mean open time of unitary N-type calcium channel currents.Neuroscience · 2010Article
- The Ca2+ channel beta subunit determines whether stimulation of Gq-coupled receptors enhances or inhibits N current.The Journal of general physiology · 2009Article
- Regulation of voltage-gated Ca2+ channels by lipids.Cell calcium · 2009Review
- Arachidonic acid inhibition of L-type calcium (CaV1.3b) channels varies with accessory CaVbeta subunits.The Journal of general physiology · 2009Article
- Arachidonic acid and ion channels: an update.British journal of pharmacology · 2008Review
- Role of PIP2 in regulating versus modulating Ca2+ channel activity.The Journal of physiology · 2007Article
- M1 muscarinic receptors inhibit L-type Ca2+ current and M-current by divergent signal transduction cascades.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Neural cell adhesion molecule induces intracellular signaling via multiple mechanisms of Ca2+ homeostasis.Molecular biology of the cell · 2006Article
- Mechanism of arachidonic acid modulation of the T-type Ca2+ channel alpha1G.The Journal of general physiology · 2004Article
- Phospholipid metabolism is required for M1 muscarinic inhibition of N-type calcium current in sympathetic neurons.European biophysics journal : EBJ · 2004Article
- Neurotransmitter modulation of neuronal calcium channels.Journal of bioenergetics and biomembranes · 2003Review
- Pharmacological discrimination between muscarinic receptor signal transduction cascades with bethanechol chloride.British journal of pharmacology · 2003Article
- Arachidonic acid mediates muscarinic inhibition and enhancement of N-type Ca2+ current in sympathetic neurons.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have characterized the actions of arachidonic acid (AA) on whole cell and unitary calcium (Ca2+) currents in rat neonatal superior cervical ganglion (SCG) neurons using barium (Ba2+) as the charge carrier. Whole cell currents were elicited by stepping the membrane potential from -90 mV to +10 mV. Arachidonic acid (5 microM) was introduced into the bath in the continued presence of 1 microM (+)-202-791, an L-type Ca2+ channel agonist. Under these conditions, the peak current, comprised mainly of N-type current, and a slow, (+)-202-791-induced component of the tail current were inhibited by 67 +/- 6 and 60 +/- 10 %, respectively, indicating that AA inhibits both N- and L-type currents. At a test potential of +30 mV, AA (5 microM) decreased unitary L- and N-type Ca2+ channel open probability (Po) in cell-attached patches that contained a single channel. For both channels, the underlying causes of the decrease in Po were similar. Arachidonic acid caused an increase in the percentage of null sweeps and in the number of null sweeps that clustered together. In sweeps with activity, the average number of openings per sweep decreased, while first latency and mean closed time increased. Arachidonic acid had no significant effect on unitary current amplitude or mean open time. Our findings are the first description of the inhibition of unitary L- and N-type Ca2+ channel activity by AA and are consistent with both channels spending more time in their null mode and with increased dwell time in one or more closed states.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.