ArticleThe Journal of physiology2000
ATP-sensitive potassium channels in capillaries isolated from guinea-pig heart.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 83 citations in OpenAlex.
- The normalized slope conductance as a tool for quantitative analysis of current-voltage relations.Biophysical journal · 2022Article
- Adenosine signaling activates ATP-sensitive KScience signaling · 2022Article
- Role of Ion Channel Remodeling in Endothelial Dysfunction Induced by Pulmonary Arterial Hypertension.Biomolecules · 2022Review
- The Potential Role of Creatine in Vascular Health.Nutrients · 2021Review
- Endothelial ATP-Sensitive Potassium Channel Protects Against the Development of Hypertension and Atherosclerosis.Hypertension (Dallas, Tex. : 1979) · 2020Article
- ATP- and voltage-dependent electro-metabolic signaling regulates blood flow in heart.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The surprising complexity of KATP channel biology and of genetic diseases.The Journal of clinical investigation · 2020Article
- Genetic Discovery of ATP-Sensitive KCirculation. Arrhythmia and electrophysiology · 2019Review
- Molecular and functional characterization of the endothelial ATP-sensitive potassium channel.The Journal of biological chemistry · 2017Article
- KATP Channels in the Cardiovascular System.Physiological reviews · 2016Review
- Cardiovascular KPathobiology of aging & age related diseases · 2016Review
- Protection of coronary endothelial function during cardiac surgery: potential of targeting endothelial ion channels in cardioprotection.BioMed research international · 2014Review
- Multiplicity of effectors of the cardioprotective agent, diazoxide.Pharmacology & therapeutics · 2013Review
- KATP channels and cardiovascular disease: suddenly a syndrome.Circulation research · 2013Review
- Iptakalim enhances adult mouse hippocampal neurogenesis via opening Kir6.1-composed K-ATP channels expressed in neural stem cells.CNS neuroscience & therapeutics · 2012Article
- Iptakalim rescues human pulmonary artery endothelial cells from hypoxia-induced nitric oxide system dysfunction.Experimental and therapeutic medicine · 2012Article
- Measuring and evaluating the role of ATP-sensitive K+ channels in cardiac muscle.Journal of molecular and cellular cardiology · 2012Review
- Iptakalim, an ATP-sensitive potassium channel opener, confers neuroprotection against cerebral ischemia/reperfusion injury in rats by protecting neurovascular unit cells.Journal of Zhejiang University. Science. B · 2011Article
- Muscle KATP channels: recent insights to energy sensing and myoprotection.Physiological reviews · 2010Review
- Localization of ATP-sensitive K+ channel subunits in rat submandibular gland.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The full-length cDNAs of two different alpha-subunits (Kir6.1 and Kir6.2) and partial cDNAs of three different beta-subunits (SUR1, SUR2A and SUR2B) of ATP-sensitive potassium (KATP) channels of the guinea-pig (gp) were obtained by screening a cDNA library from the ventricle of guinea-pig heart. Cell-specific reverse-transcriptase PCR with gene-specific intron-spanning primers showed that gpKir6.1, gpKir6.2 and gpSUR2B were expressed in a purified fraction of capillary endothelial cells. In cardiomyocytes, gpKir6.1, gpKir6.2, gpSUR1 and gpSUR2A were detected. Patch-clamp measurements were carried out in isolated capillary fragments consisting of 3-15 endothelial cells. The membrane capacitance measured in the whole-cell mode was 19.9 +/- 1.0 pF and was independent of the length of the capillary fragment, which suggests that the endothelial cells were not electrically coupled under our experimental conditions. The perforated-patch technique was used to measure the steady-state current-voltage relation of capillary endothelial cells. Application of K+ channel openers (rilmakalim or diazoxide) or metabolic inhibition (250 microM 2,4-dinitrophenol plus 10 mM deoxyglucose) induced a current that reversed near the calculated K+ equilibrium potential. Rilmakalim (1 microM), diazoxide (300 microM) and metabolic inhibition increased the slope conductance measured at -55 mV by a factor of 9.0 (+/-1.8), 2.5 (+/-0.2) and 3.9 (+/-1.7), respectively. The effects were reversed by glibenclamide (1 microM). Our results suggest that capillary endothelial cells from guinea-pig heart express KATP channels composed of SUR2B and Kir6.1 and/or Kir6.2 subunits. The hyperpolarization elicited by the opening of KATP channels may lead to an increase in free cytosolic Ca2+, and thus modulate the synthesis of NO and the permeability of the capillary wall.
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