ArticleJournal of clinical microbiology2000
Extraction-free, filter-based template preparation for rapid and sensitive PCR detection of pathogenic parasitic protozoa.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
53 citing papers in PubMed, 158 citations in OpenAlex.
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- A robust, hand-powered, instrument-free sample preparation system for point-of-care pathogen detection.Scientific reports · 2019Article
- Intestinal parasitism in pediatric oncology children receiving chemotherapy: unexpected low prevalence.Heliyon · 2019Article
- Enteric Opportunistic Infection and the Impact of Antiretroviral Therapy among HIV/AIDS Patients from Tehran, Iran.Iranian journal of public health · 2019Article
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- An overview of methods/techniques for the detection of Cryptosporidium in food samples.Parasitology research · 2018Review
- Microbial pollution and food safety.AIMS microbiology · 2018Review
- Simultaneous Molecular Detection ofThe Korean journal of parasitology · 2017Article
- Multilocus sequence typing ofThe Indian journal of medical research · 2017Article
- The current state of knowledge on the interaction ofEnergy, ecology & environment · 2016Article
- Molecular appraisal of intestinal parasitic infection in transplant recipients.The Indian journal of medical research · 2016Article
- Intestinal Parasitosis in Relation to Anti-Retroviral Therapy, CD4(+) T-cell Count and Diarrhea in HIV Patients.The Korean journal of parasitology · 2015Article
- Detection of enteropathogens associated with travelers' diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards.Diagnostic microbiology and infectious disease · 2015Article
- Detection of Nosema bombycis by FTA cards and loop-mediated isothermal amplification (LAMP).Current microbiology · 2014Article
- Molecular Identification of Giardia duodenalis Isolates from Fars Province, Iran.Iranian journal of parasitology · 2014Article
- Prevalence and genetic characterization of Cryptosporidium, Enterocytozoon, Giardia and Cyclospora in diarrheal outpatients in China.BMC infectious diseases · 2014Article
- Modified Negative Staining of Heine for Fast and Inexpensive Screening of Cryptosporidium, Cyclospora, and Cystoisospora spp.International scholarly research notices · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Within the last several years, the protozoan parasites Cyclospora cayetanensis, Cryptosporidium parvum, and microsporidia have become recognized as important, rapidly emerging human pathogens in immunocompromised and immunocompetent individuals. Since the early 1990s, many of the reported outbreaks of enteric illness caused by these microorganisms have been attributed to food- and water-borne contamination. Many inherent obstacles affect the success of current surveillance and detection methods used to monitor and control levels of contamination by these pathogens. Unlike methods that incorporate preenrichment for easier and unambiguous identification of bacterial pathogens, similar methods for the detection of parasitic protozoa either are not currently available or cannot be performed in a timely manner. We have developed an extraction-free, filter-based protocol to prepare DNA templates for use in PCR to identify C. cayetanensis and C. parvum oocysts and microsporidia spores. This method requires only minimal preparation to partially purify and concentrate isolates prior to filter application. DNA template preparation is rapid, efficient, and reproducible. As few as 3 to 10 parasites could be detected by PCR from direct application to the filters. In studies, as few 10 to 50 Encephalitozoon intestinalis spores could be detected when seeded in a 100-microliter stool sample and 10 to 30 C. cayetanensis oocysts could be detected per 100 g of fresh raspberries. This protocol can easily be adapted to detect parasites from a wide variety of food, clinical, and environmental samples and can be used in multiplex PCR applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.