ArticleJournal of clinical microbiology2000
PCR and restriction endonuclease analysis for rapid identification of human adenovirus subgenera.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 47 citations in OpenAlex.
- Virucidal Action Mechanism of Alcohol and Divalent Cations Against Human Adenovirus.Frontiers in molecular biosciences · 2020Article
- Prevalence of respiratory adenovirus species B and C in sewage sludge.Environmental science. Processes & impacts · 2013Article
- Molecular Classification of Human Adenovirus Type 7 Isolated From Acute Respiratory Disease Outbreak (ARD) in Korea, 2005-2006.Osong public health and research perspectives · 2010Article
- Molecular identification of adenovirus causing respiratory tract infection in pediatric patients at the University of Malaya Medical Center.BMC pediatrics · 2010Article
- Outbreak of epidemic keratoconjunctivitis caused by adenovirus in medical residents.Molecular vision · 2009Article
- Quantitative detection and rapid identification of human adenoviruses.Journal of clinical microbiology · 2007Article
- Molecular identification of adenoviruses in clinical samples by analyzing a partial hexon genomic region.Journal of clinical microbiology · 2005Article
- Molecular characterization of adeno-associated viruses infecting children.Journal of virology · 2005Article
- Rapid identification of human adenovirus types 3 and 7 from respiratory specimens via multiplex type-specific PCR.Journal of clinical microbiology · 2005Article
- Three nonfatal cases of neonatal adenovirus infection.Journal of clinical microbiology · 2005Article
- Multiplex PCR assay for rapid identification of oculopathogenic adenoviruses by amplification of the fiber and hexon genes.Journal of clinical microbiology · 2005Article
- Development of a PCR-and hybridization-based assay (PCR Adenovirus Consensus) for the detection and the species identification of adenoviruses in respiratory specimens.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2004Article
- Comprehensive detection and serotyping of human adenoviruses by PCR and sequencing.Journal of clinical microbiology · 2004Article
- Rapid detection and identification of human adenovirus species by adenoplex, a multiplex PCR-enzyme hybridization assay.Journal of clinical microbiology · 2004Article
- Use of oligonucleotide microarrays for rapid detection and serotyping of acute respiratory disease-associated adenoviruses.Journal of clinical microbiology · 2004Article
- Molecular diagnosis of human adenoviruses d and e by a phylogeny-based classification method using a partial hexon sequence.Journal of clinical microbiology · 2004Article
- Genotyping of enteric adenoviruses by using single-stranded conformation polymorphism analysis and heteroduplex mobility assay.Journal of clinical microbiology · 2004Article
- Identification of subgenus C adenoviruses by fiber-based multiplex PCR.Journal of clinical microbiology · 2004Article
- Identification of the hexon region of an adenovirus involved in a new outbreak of keratoconjunctivitis.Journal of clinical microbiology · 2001Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Subgenus identification of adenoviruses is of clinical importance and is as informative as identification by serotype in most clinical situations. A PCR-based identification of adenovirus subgenera A, B, C, D, E, and F and sometimes serotypes is described. The PCR uses nonnested primer pair ADRJC1-ADRJC2, which targets a highly conserved region of the adenovirus hexon gene, has a sensitivity of 10 to 40 copies of adenovirus type 2 (Ad2) DNA, and generates 140-bp PCR products from adenovirus serotypes representative of all the subgroups. The PCR products of all subgroups can be differentiated on the basis of the restriction fragment patterns produced by a total of five restriction endonucleases. In addition, serotypes Ad40 and Ad41 (subgroup F) and important serotypes of subgroup D (Ad8, Ad10, Ad19, and Ad37) can easily be differentiated, but serotypes within subgroups B and C cannot. The method was assessed by blind subgenus identification of 56 miscellaneous clinical isolates of adenoviruses. The identities of these isolates at the subgenus level by the PCR correlated 91% (51 of 56) with the results of serotyping by the neutralization test, and 9% (5 of 56) of clinical isolates produced discordant results.
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