ArticleThe Journal of biological chemistry2000
Palmitoylation of caveolin-1 is required for cholesterol binding, chaperone complex formation, and rapid transport of cholesterol to caveolae.
Article in The Journal of biological chemistry, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 58 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
58 citing papers in PubMed, 174 citations in OpenAlex.
- MAVS Cys508 palmitoylation promotes its aggregation on the mitochondrial outer membrane and antiviral innate immunity.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Caveolin-1 deficiency impairs synaptic transmission in hippocampal neurons.Molecular brain · 2021Article
- Caveolin-1 and MLRs: A potential target for neuronal growth and neuroplasticity after ischemic stroke.International journal of medical sciences · 2019Review
- Caveolin-1: An Oxidative Stress-Related Target for Cancer Prevention.Oxidative medicine and cellular longevity · 2017Review
- From embryonic development to human diseases: The functional role of caveolae/caveolin.Birth defects research. Part C, Embryo today : reviews · 2016Review
- There Is No Simple Model of the Plasma Membrane Organization.Frontiers in cell and developmental biology · 2016Review
- Caveolae, caveolin-1 and cavin-1: Emerging roles in pulmonary hypertension.World journal of respirology · 2015Article
- Function of Integrin-Linked Kinase in Modulating the Stemness of IL-6-Abundant Breast Cancer Cells by Regulating γ-Secretase-Mediated Notch1 Activation in Caveolae.Neoplasia (New York, N.Y.) · 2015Article
- Ezetimibe suppresses cholesterol accumulation in lipid-loaded vascular smooth muscle cells in vitro via MAPK signaling.Acta pharmacologica Sinica · 2014Article
- Channelopathies from mutations in the cardiac sodium channel protein complex.Journal of molecular and cellular cardiology · 2013Review
- Chaperone properties of pdia3 participate in rapid membrane actions of 1α,25-dihydroxyvitamin d3.Molecular endocrinology (Baltimore, Md.) · 2013Article
- Palmitoylation of MPP1 (membrane-palmitoylated protein 1)/p55 is crucial for lateral membrane organization in erythroid cells.The Journal of biological chemistry · 2012Article
- Probing the caveolin-1 P132L mutant: critical insights into its oligomeric behavior and structure.Biochemistry · 2012Article
- The transmembrane domain of caveolin-1 exhibits a helix-break-helix structure.Biochimica et biophysica acta · 2012Article
- Regulation of cholesterol homeostasis.Cellular and molecular life sciences : CMLS · 2012Review
- Structural characterization of the caveolin scaffolding domain in association with cholesterol-rich membranes.Biochemistry · 2012Article
- Article
- Caveolin-1 orchestrates TCR synaptic polarity, signal specificity, and function in CD8 T cells.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Cholesterol esters as growth regulators of lymphocytic leukaemia cells.Cell proliferation · 2011Article
- Cholesterol asymmetry in synaptic plasma membranes.Journal of neurochemistry · 2011Article
Corrections and comments
- Retraction · 2013-03-01Falsification/Fabrication of Image · Investigation by Company/Institution · Investigation by ORI · Misconduct - Official Investigation(s) and/or Finding(s) · Misconduct by Author · · NIH misconduct finding for Eric J Smart
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- Retracted
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We previously demonstrated that a caveolin-chaperone complex transports newly synthesized cholesterol from the endoplasmic reticulum through the cytoplasm to caveolae. Caveolin-1 has a 33-amino acid hydrophobic domain and three sites of palmitoylation in proximity to the hydrophobic domain. In the present study, we hypothesized that palmitoylation of caveolin-1 is necessary for binding of cholesterol, formation of a caveolin-chaperone transport complex, and rapid, direct transport of cholesterol to caveolae. To test this hypothesis, four caveolin-1 constructs were generated that substituted an alanine for a cysteine at position 133, 143, or 156 or all three sites (triple mutant). These mutated caveolins and wild type caveolin-1 were stably expressed in the lymphoid cell line, L1210-JF, which does not express caveolin-1, does not form a caveolin-chaperone complex, and does not transport newly synthesized cholesterol to caveolae. All of the caveolins were expressed and the proteins localized to plasma membrane caveolae. Wild type caveolin-1 and mutant 133 assembled into complete transport complexes and rapidly (10-20 min) transported cholesterol to caveolae. Caveolin mutants 143 and 156 did not assemble into complete transport complexes, weakly associated with cholesterol, and transported small amounts of cholesterol to caveolae. The triple mutant did not assemble into complete transport complexes and did not associate with cholesterol. We conclude that palmitoylation of caveolin-1 at positions 143 and 156 is required for cholesterol binding and transport complex formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.