ArticleProceedings of the National Academy of Sciences of the United States of America2000
Analysis of telomerase catalytic subunit mutants in vivo and in vitro in Schizosaccharomycespombe.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 54 citations in OpenAlex.
- Fission yeast RPA-TERT-Tpz1TPP1 complex promotes telomere extension and suppresses telomere recombination.PLoS genetics · 2026Article
- Fission yeast RPA-TERT-Tpz1bioRxiv : the preprint server for biology · 2026Article
- Crystal structures of N-terminally truncated telomerase reverse transcriptase from fungi‡.Nucleic acids research · 2021Article
- RNAi drives nonreciprocal translocations at eroding chromosome ends to establish telomere-free linear chromosomes.Genes & development · 2018Article
- LARP7-like protein Pof8 regulates telomerase assembly and poly(A)+TERRA expression in fission yeast.Nature communications · 2018Article
- Analysis of Yeast Telomerase by Primer Extension Assays.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Telomerase RNA stem terminus element affects template boundary element function, telomere sequence, and shelterin binding.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Fission yeast shelterin regulates DNA polymerases and Rad3(ATR) kinase to limit telomere extension.PLoS genetics · 2013Article
- Article
- It all comes together at the ends: telomerase structure, function, and biogenesis.Mutation research · 2012Review
- Article
- InTERTpreting telomerase structure and function.Nucleic acids research · 2010Review
- Roles of heterochromatin and telomere proteins in regulation of fission yeast telomere recombination and telomerase recruitment.The Journal of biological chemistry · 2010Article
- Telomeres and disease.The EMBO journal · 2009Review
- A flexible template boundary element in the RNA subunit of fission yeast telomerase.The Journal of biological chemistry · 2008Article
- Recombination-based telomere maintenance is dependent on Tel1-MRN and Rap1 and inhibited by telomerase, Taz1, and Ku in fission yeast.Molecular and cellular biology · 2008Article
- Identification and characterization of the Schizosaccharomyces pombe TER1 telomerase RNA.Nature structural & molecular biology · 2008Article
- Characterization of recombinant Saccharomyces cerevisiae telomerase core enzyme purified from yeast.The Biochemical journal · 2005Article
- Two modes of DNA double-strand break repair are reciprocally regulated through the fission yeast cell cycle.Genes & development · 2004Article
- Replication proteins influence the maintenance of telomere length and telomerase protein stability.Molecular and cellular biology · 2003Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The chromosome end-replicating enzyme telomerase is composed of a template-containing RNA subunit, a reverse transcriptase (TERT), and additional proteins. The importance of conserved amino acid residues in Trt1p, the TERT of Schizosaccharomyces pombe, was tested. Mutation to alanine of the proposed catalytic aspartates in reverse transcriptase motifs A and C and of conserved amino acids in motifs 1 and B' resulted in defective growth, progressive loss of telomeric DNA, and loss of detectable telomerase enzymatic activity in vitro. Mutation of the phenylalanine (F) in the conserved FYxTE of telomerase-specific motif T had no phenotype in vivo or in vitro whereas mutation of a conserved amino acid in RT motif 2 had an intermediate effect. In addition to identifying single amino acids of TERT required for telomere maintenance in the fission yeast, this work provides useful tools for S. pombe telomerase research: a functional epitope-tagged version of Trt1p that allows detection of the protein even in crude cellular extracts, and a convenient and robust in vitro enzymatic activity assay based on immunopurification of telomerase.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.