ArticleThe Journal of general physiology2000
Interactions of cyclic nucleotide-gated channel subunits and protein tyrosine kinase probed with genistein.
Article in The Journal of general physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Voltage-dependent opening of HCN channels: Facilitation or inhibition by the phytoestrogen, genistein, is determined by the activation status of the cyclic nucleotide gating ring.Biochimica et biophysica acta · 2009Article
- Article
- Native cone photoreceptor cyclic nucleotide-gated channel is a heterotetrameric complex comprising both CNGA3 and CNGB3: a study using the cone-dominant retina of Nrl-/- mice.Journal of neurochemistry · 2008Article
- Tyrosine phosphorylation of cGMP-gated ion channels is under circadian control in chick retina photoreceptors.Investigative ophthalmology & visual science · 2007Article
- Effects of the protein tyrosine kinase inhibitor genistein and taurine on retinal function in isolated superfused retina.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2007Article
- Lateral hypothalamic signaling mechanisms underlying feeding stimulation: differential contributions of Src family tyrosine kinases to feeding triggered either by NMDA injection or by food deprivation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Subunit contributions to phosphorylation-dependent modulation of bovine rod cyclic nucleotide-gated channels.The Journal of physiology · 2003Article
- Potentiation of 'on' bipolar cell flash responses by dim background light and cGMP in dogfish retinal slices.The Journal of physiology · 2002Article
- Rectification of cGMP-activated channels induced by phosphorylation in dogfish retinal 'on' bipolar cells.The Journal of physiology · 2001Article
- Mechanism of inhibition of cyclic nucleotide-gated channel by protein tyrosine kinase probed with genistein.The Journal of general physiology · 2001Article
- Developmental expression of retinal cone cGMP-gated channels: evidence for rapid turnover and trophic regulation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The cGMP sensitivity of cyclic nucleotide-gated (CNG) channels can be modulated by changes in phosphorylation catalyzed by protein tyrosine kinases (PTKs) and protein tyrosine phosphatases. Previously, we used genistein, a PTK inhibitor, to probe the interaction between PTKs and homomeric channels comprised of alpha subunits (RETalpha) of rod photoreceptor CNG channels expressed in Xenopus oocytes. We showed that in addition to inhibiting phosphorylation, genistein triggers a noncatalytic interaction between PTKs and homomeric RETalpha channels that allosterically inhibits channel gating. Here, we show that native CNG channels from rods, cones, and olfactory receptor neurons also exhibit noncatalytic inhibition induced by genistein, suggesting that in each of these sensory cells, CNG channels are part of a regulatory complex that contains PTKs. Native CNG channels are heteromers, containing beta as well as alpha subunits. To determine the contributions of alpha and beta subunits to genistein inhibition, we compared the effect of genistein on native, homomeric (RETalpha and OLFalpha), and heteromeric (RETalpha+beta, OLFalpha+beta, and OLFalpha+RETbeta) CNG channels. We found that genistein only inhibits channels that contain either the RETalpha or the OLFbeta subunits. This finding, along with other observations about the maximal effect of genistein and the Hill coefficient of genistein inhibition, suggests that the RETalpha and OLFbeta subunits contain binding sites for the PTK, whereas RETbeta and OLFalpha subunits do not.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.