ArticleBiophysical journal2000
Comparative single-molecule and ensemble myosin enzymology: sulfoindocyanine ATP and ADP derivatives.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- A FRET assay to monitor different structural states of human β-cardiac myosin including the interacting-heads motif.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Single molecule turnover of fluorescent ATP by myosin and actomyosin unveil elusive enzymatic mechanisms.Communications biology · 2021Article
- The Kinetics of Nucleotide Binding to Isolated Chlamydomonas Axonemes Using UV-TIRF Microscopy.Biophysical journal · 2019Article
- Direct visualization of human myosin II force generation using DNA origami-based thick filaments.Communications biology · 2019Article
- Single molecule detection, thermal fluctuation and life.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2017Review
- ATP turnover by individual myosin molecules hints at two conformers of the myosin active site.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Single-molecule fluorescence imaging of processive myosin with enhanced background suppression using linear zero-mode waveguides (ZMWs) and convex lens induced confinement (CLIC).Optics express · 2013Article
- Single turnovers of fluorescent ATP bound to bipolar myosin filament during actin filaments sliding.Biophysics (Nagoya-shi, Japan) · 2013Article
- Controlled rotation of the F₁-ATPase reveals differential and continuous binding changes for ATP synthesis.Nature communications · 2012Article
- Simultaneous measurement of nucleotide occupancy and mechanical displacement in myosin-V, a processive molecular motor.Biophysical journal · 2009Article
- Direct observation of the mechanochemical coupling in myosin Va during processive movement.Nature · 2008Article
- Mechanical properties of inner-arm dynein-f (dynein I1) studied with in vitro motility assays.Biophysical journal · 2007Article
- Synthesis and fluorescence studies of some new fluorophores and their effect on hybridization of oligodeoxyribonucleotides.Journal of fluorescence · 2007Article
- Higher plant myosin XI moves processively on actin with 35 nm steps at high velocity.The EMBO journal · 2003Article
- The 2'-O- and 3'-O-Cy3-EDA-ATP(ADP) complexes with myosin subfragment-1 are spectroscopically distinct.Biophysical journal · 2003Article
- Combined single-molecule force and fluorescence measurements for biology.Journal of biology · 2003Review
- Single-molecule investigation of the interference between kinesin, tau and MAP2c.The EMBO journal · 2002Article
- A novel pressure-jump apparatus for the microvolume analysis of protein-ligand and protein-protein interactions: its application to nucleotide binding to skeletal-muscle and smooth-muscle myosin subfragment-1.The Biochemical journal · 2002Article
- The biochemical kinetics underlying actin movement generated by one and many skeletal muscle myosin molecules.Biophysical journal · 2002Article
- Fluorescent coumarin-labeled nucleotides to measure ADP release from actomyosin.Biophysical journal · 2001Article
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-molecule and macroscopic reactions of fluorescent nucleotides with myosin have been compared. The single-molecule studies serve as paradigms for enzyme-catalyzed reactions and ligand-receptor interactions analyzed as individual stochastic processes. Fluorescent nucleotides, called Cy3-EDA-ATP and Cy5-EDA-ATP, were derived by coupling the dyes Cy3.29.OH and Cy5.29.OH (compounds XI and XIV, respectively, in, Bioconjug. Chem. 4:105-111)) with 2'(3')-O-[N-(2-aminoethyl)carbamoyl]ATP (EDA-ATP). The ATP(ADP) analogs were separated into their respective 2'- and 3'-O-isomers, the interconversion rate of which was 30[OH(-)] s(-1) (0.016 h(-1) at pH 7.1) at 22 degrees C. Macroscopic studies showed that 2'(3')-O-substituted nucleotides had properties similar to those of ATP and ADP in their interactions with myosin, actomyosin, and muscle fibers, although the ATP analogs did not relax muscle as well as ATP did. Significant differences in the fluorescence intensity of Cy3-nucleotide 2'- and 3'-O-isomers in free solution and when they interacted with myosin were evident. Single-molecule studies using total internal reflection fluorescence microscopy showed that reciprocal mean lifetimes of the nucleotide analogs interacting with myosin filaments were one- to severalfold greater than predicted from macroscopic data. Kinetic and equilibrium data of nucleotide-(acto)myosin interactions derived from single-molecule microscopy now have a biochemical and physiological framework. This is important for single-molecule mechanical studies of motor proteins.
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