ArticleBiophysical journal2000
Resolution of patch capacitance recordings and of fusion pore conductances in small vesicles.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed.
- Fusion Pores as Regulators of Quantal Size and Cellular Physiology.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Multiple Modes of Fusion and Retrieval at the Calyx of Held Synapse.Advances in neurobiology · 2023Article
- Patch Amperometry and Intracellular Patch Electrochemistry.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Reconstitution and real-time quantification of membrane remodeling by single proteins and protein complexes.Nature protocols · 2020Article
- Phosphatidylserine is critical for vesicle fission during clathrin-mediated endocytosis.Journal of neurochemistry · 2020Article
- Individual synaptic vesicles mediate stimulated exocytosis from cochlear inner hair cells.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The fusion pore, 60 years after the first cartoon.FEBS letters · 2018Review
- Toward a unified picture of the exocytotic fusion pore.FEBS letters · 2018Review
- Positively charged amino acids at the SNAP-25 C terminus determine fusion rates, fusion pore properties, and energetics of tight SNARE complex zippering.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Methods for cell-attached capacitance measurements in mouse adrenal chromaffin cell.Journal of visualized experiments : JoVE · 2014Article
- A new role for myosin II in vesicle fission.PloS one · 2014Article
- Actin polymerization does not provide direct mechanical forces for vesicle fission during clathrin-mediated endocytosis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- High-resolution membrane capacitance measurements for the study of exocytosis and endocytosis.Nature protocols · 2013Article
- cAMP-mediated stabilization of fusion pores in cultured rat pituitary lactotrophs.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Juxtamembrane tryptophans of synaptobrevin 2 control the process of membrane fusion.FEBS letters · 2013Article
- High resolution electrophysiological techniques for the study of calcium-activated exocytosis.Biochimica et biophysica acta · 2012Review
- Synaptotagmin 1 is necessary for the Ca2+ dependence of clathrin-mediated endocytosis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Permeation of styryl dyes through nanometer-scale pores in membranes.Biochemistry · 2011Article
- Munc18-1 tuning of vesicle merger and fusion pore properties.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Capacitance measurements of regulated exocytosis in mouse taste cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the noise levels in cell-attached patch capacitance recordings with a lock-in amplifier. The capacitance noise level decreases with increasing sine wave frequency up to 20-40 kHz. With a 20-mV rms sine wave the rms noise level above 8 kHz is <50 aF. With increasing sine wave amplitudes a further reduction down to 14 aF could be achieved. Capacitance measurements with a lock-in amplifier may also be used to measure the conductance of fusion pores connecting the vesicular lumen to the extracellular space. It is estimated that at noise levels of 14 aF fusion pore conductances between 20 pS and 700 pS may be resolved in vesicles with 380-aF capacitance by using a 50-kHz sine wave. This corresponds to vesicles with a approximately 110-nm diameter. It is suggested that with low-noise techniques fusion pores may be detectable in vesicles approaching the size of large synaptic vesicles.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.