ArticleMolecular and cellular biology2000
A zinc finger transcription factor, alphaA-crystallin binding protein 1, is a negative regulator of the chondrocyte-specific enhancer of the alpha1(II) collagen gene.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 53 citations in OpenAlex.
- Chondrocytes-Specific Expression of Osteoprotegerin Modulates Osteoclast Formation in Metaphyseal Bone.Scientific reports · 2015Article
- Col2CreER(T2), a mouse model for a chondrocyte-specific and inducible gene deletion.European cells & materials · 2014Review
- Uncoupling of growth plate maturation and bone formation in mice lacking both Schnurri-2 and Schnurri-3.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Arginine methyltransferase CARM1/PRMT4 regulates endochondral ossification.BMC developmental biology · 2009Article
- Study of the association between polymorphisms of the COL1A1 gene and HBV-related liver cirrhosis in Chinese patients.Digestive diseases and sciences · 2009Article
- Inhibition of beta-catenin signaling causes defects in postnatal cartilage development.Journal of cell science · 2008Article
- Schnurri-3 is an essential regulator of osteoblast function and adult bone mass.Annals of the rheumatic diseases · 2007Review
- Generation of a transgenic mouse model with chondrocyte-specific and tamoxifen-inducible expression of Cre recombinase.Genesis (New York, N.Y. : 2000) · 2007Article
- Reciprocal regulation of nuclear factor kappa B and its inhibitor ZAS3 after peripheral nerve injury.BMC neuroscience · 2006Article
- Transcriptional coactivator PGC-1alpha regulates chondrogenesis via association with Sox9.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Oligonucleotide decoy mimicking alphaA-crystallin-binding protein 1 binding site on mouse Col2a1 enhancer stimulates transcription from the adjacent Col2a1 promoter in chondrogenic ATDC5 cell.Molecular biotechnology · 2004Article
- Transcriptional regulation of human alpha1(I) procollagen gene in dermal fibroblasts.World journal of gastroenterology · 2004Article
- Evaluation of different conditions for ligating dumbbell-shaped oligonucleotides.Molecular biotechnology · 2004Article
- Mouse Snail family transcription repressors regulate chondrocyte, extracellular matrix, type II collagen, and aggrecan.The Journal of biological chemistry · 2003Article
- Inhibition of NF-kappaB by ZAS3, a zinc-finger protein that also binds to the kappaB motif.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- The kappa B transcriptional enhancer motif and signal sequences of V(D)J recombination are targets for the zinc finger protein HIVEP3/KRC: a site selection amplification binding study.BMC immunology · 2002Article
- A Krüppel-associated box-zinc finger protein, NT2, represses cell-type-specific promoter activity of the alpha 2(XI) collagen gene.Molecular and cellular biology · 2002Article
- Expression of chondrocyte markers by cells of normal and degenerate intervertebral discs.Molecular pathology : MP · 2002Article
- ZAS: C2H2 zinc finger proteins involved in growth and development.Gene expression · 2002Review
- Could inflammatory arthritis be triggered by progenitor cells in the joints?Annals of the rheumatic diseases · 2002Review
Corrections and comments
- Retraction · 2009-06-01Concerns/Issues about Image · Duplication of/in Image · Falsification/Fabrication of Data · Falsification/Fabrication of Image · Investigation by ORI · Investigation by Third Party · Misconduct - Official Investigation(s) and/or Finding(s) · Misconduct by Author · Upgrade/Update of Prior Notice(s) · · A 'Finding of scientific misconduct' relating to this paper is available here: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259699/; The publisher retracts both the original article and the 'partial retraction' [of images] from 2006.;
- Correction · 2006-07-01Concerns/Issues about Image · Updated to Retraction · · This correction retracts 2 figures used in the original article.;
- Commented on by
- Erratum issued
- RetractedRetraction.2009
Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription of the type II collagen gene (Col2a1) is regulated by multiple cis-acting sites. The enhancer element, which is located in the first intron, is necessary for high-level and cartilage-specific expression of Col2a1. A mouse limb bud cDNA expression library was screened by the Saccharomyces cerevisiae one-hybrid screening method to identify protein factors bound to the enhancer. A zinc finger protein, alphaA-crystallin binding protein 1 (CRYBP1), which had been reported to bind to the mouse alphaA-crystallin gene promoter, was isolated. We herein demonstrate that CRYBP1 is involved in the negative regulation of Col2a1 enhancer activity. CRYBP1 mRNA expression was downregulated during chondrocyte differentiation in vitro. In situ hybridization analysis of developing mouse cartilage showed that CRYBP1 mRNA was also downregulated during mesenchymal condensation and that CRYBP1 mRNA was highly expressed by hypertrophic chondrocytes, but at very low levels by resting and proliferating chondrocytes. Expression of recombinant CRYBP1 in a transfected rat chondrosarcoma cell line inhibited Col2a1 enhancer activity. Electrophoretic mobility shift assays showed that CRYBP1 bound a specific sequence within the Col2a1 enhancer and inhibited the binding of Sox9, an activator for Col2a1, to the enhancer. Cotransfection of CRYBP1 with Sox9 into BALB/c 3T3 cells inhibited activation of the Col2a1 enhancer by Sox9. These results suggest a novel mechanism that negatively regulates cartilage-specific expression of Col2a1.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.