ArticleMolecular and cellular biology2000
Mechanism for specificity by HMG-1 in enhanceosome assembly.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 87 citations in OpenAlex.
- Modulation of α7nAchR by Melatonin Alleviates Ischemia and Reperfusion-Compromised Integrity of Blood-Brain Barrier Through Inhibiting HMGB1-Mediated Microglia Activation and CRTC1-Mediated Neuronal Loss.Cellular and molecular neurobiology · 2022Article
- Article
- Transcriptional Enhancers inGenetics · 2020Review
- Detrimental Effects of HMGB-1 Require Microglial-Astroglial Interaction: Implications for the Status Epilepticus -Induced Neuroinflammation.Frontiers in cellular neuroscience · 2019Article
- Pivotal neuroinflammatory and therapeutic role of high mobility group box 1 in ischemic stroke.Bioscience reports · 2017Review
- Deciphering the role of the AT-rich interaction domain and the HMG-box domain of ARID-HMG proteins of Arabidopsis thaliana.Plant molecular biology · 2016Article
- Mitochondrial Translocation of High Mobility Group Box 1 Facilitates LIM Kinase 2-Mediated Programmed Necrotic Neuronal Death.Frontiers in cellular neuroscience · 2016Article
- Role of nucleosome remodeling in neurodevelopmental and intellectual disability disorders.Frontiers in behavioral neuroscience · 2015Review
- HMGB1 in health and disease.Molecular aspects of medicine · 2014Review
- Role of the acidic tail of high mobility group protein B1 (HMGB1) in protein stability and DNA bending.PloS one · 2013Article
- High-mobility group box 1: an amplifier of stem and progenitor cell activity after stroke.Acta neurochirurgica. Supplement · 2013Review
- Nucleosome dynamics: HMGB1 relaxes canonical nucleosome structure to facilitate estrogen receptor binding.Nucleic acids research · 2012Article
- Basic N-terminus of yeast Nhp6A regulates the mechanism of its DNA flexibility enhancement.Journal of molecular biology · 2012Article
- Understanding apparent DNA flexibility enhancement by HU and HMGB architectural proteins.Journal of molecular biology · 2011Article
- The role of response elements organization in transcription factor selectivity: the IFN-β enhanceosome example.PLoS computational biology · 2011Article
- Expanding the paradigm for estrogen receptor binding and transcriptional activation.Molecular endocrinology (Baltimore, Md.) · 2011Article
- HMBG1 mediates ischemia-reperfusion injury by TRIF-adaptor independent Toll-like receptor 4 signaling.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2011Article
- Biphasic actions of HMGB1 signaling in inflammation and recovery after stroke.Annals of the New York Academy of Sciences · 2010Review
- High-mobility group protein box-1 and its relevance to cerebral ischemia.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2010Review
- Enhancement of DNA flexibility in vitro and in vivo by HMGB box A proteins carrying box B residues.Biochemistry · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Assembly of enhanceosomes requires architectural proteins to facilitate the DNA conformational changes accompanying cooperative binding of activators to a regulatory sequence. The architectural protein HMG-1 has been proposed to bind DNA in a sequence-independent manner, yet, paradoxically, it facilitates specific DNA binding reactions in vitro. To investigate the mechanism of specificity we explored the effect of HMG-1 on binding of the Epstein-Barr virus activator ZEBRA to a natural responsive promoter in vitro. DNase I footprinting, mutagenesis, and electrophoretic mobility shift assay reveal that HMG-1 binds cooperatively with ZEBRA to a specific DNA sequence between two adjacent ZEBRA recognition sites. This binding requires a strict alignment between two adjacent ZEBRA sites and both HMG boxes of HMG-1. Our study provides the first demonstration of sequence-dependent binding by a nonspecific HMG-box protein. We hypothesize how a ubiquitous, nonspecific architectural protein can function in a specific context through the use of rudimentary sequence recognition coupled with cooperativity. The observation that an abundant architectural protein can bind DNA cooperatively and specifically has implications towards understanding HMG-1's role in mediating DNA transactions in a variety of enzymological systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.