ArticleMolecular and cellular biology2000
Artificial recruitment of TFIID, but not RNA polymerase II holoenzyme, activates transcription in mammalian cells.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Determinants of transcription factor regulatory range.Nature communications · 2020Article
- The evolution of the 9aaTAD domain in Sp2 proteins: inactivation with valines and intron reservoirs.Cellular and molecular life sciences : CMLS · 2020Article
- Role of transcription complexes in the formation of the basal methylation pattern in early development.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The 9aaTAD Is Exclusive Activation Domain in Gal4.PloS one · 2017Article
- Activation of silenced cytokine gene promoters by the synergistic effect of TBP-TALE and VP64-TALE activators.PloS one · 2014Article
- Enforcing the pause: transcription factor Sp3 limits productive elongation by RNA polymerase II.Cell cycle (Georgetown, Tex.) · 2013Article
- Transition step during assembly of HIV Tat:P-TEFb transcription complexes and transfer to TAR RNA.Molecular and cellular biology · 2012Article
- The transcription-repression domain of the adenovirus E1A oncoprotein targets p300 at the promoter.Oncogene · 2008Article
- Artificial recruitment of mediator by the DNA-binding domain of Adr1 overcomes glucose repression of ADH2 expression.Molecular and cellular biology · 2008Article
- The transition from transcriptional initiation to elongation.Current opinion in genetics & development · 2008Review
- Bypassing the requirements for epigenetic modifications in gene transcription by increasing enhancer strength.Molecular and cellular biology · 2008Article
- Specific TATAA and bZIP requirements suggest that HTLV-I Tax has transcriptional activity subsequent to the assembly of an initiation complex.Retrovirology · 2004Article
- Molecular requirements for gene expression mediated by targeted histone acetyltransferases.Molecular and cellular biology · 2004Article
- TFIIB-facilitated recruitment of preinitiation complexes by a TAF-independent mechanism.Nucleic acids research · 2004Article
- Human mediator enhances activator-facilitated recruitment of RNA polymerase II and promoter recognition by TATA-binding protein (TBP) independently of TBP-associated factors.Molecular and cellular biology · 2003Article
- Antithetic effects of MBD2a on gene regulation.Molecular and cellular biology · 2003Article
- Androgen-induced recruitment of RNA polymerase II to a nuclear receptor-p160 coactivator complex.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Mutations in the histone fold domain of the TAF12 gene show synthetic lethality with the TAF1 gene lacking the TAF N-terminal domain (TAND) by different mechanisms from those in the SPT15 gene encoding the TATA box-binding protein (TBP).Nucleic acids research · 2003Article
- Signal-induced transcriptional activation by Dif requires the dTRAP80 mediator module.Molecular and cellular biology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In yeast cells, transcriptional activation occurs when the RNA polymerase II (Pol II) machinery is artificially recruited to a promoter by fusing individual components of this machinery to a DNA-binding domain. Here, we show that artificial recruitment of components of the TFIID complex can activate transcription in mammalian cells. Surprisingly, artificial recruitment of TATA-binding protein (TBP) activates transiently transfected and chromosomally integrated promoters with equal efficiency, whereas artificial recruitment of TBP-associated factors activates only chromosomal reporters. In contrast, artificial recruitment of various components of the mammalian Pol II holoenzyme does not confer transcriptional activation, nor does it result in synergistic activation in combination with natural activation domains. In the one case examined in more detail, the Srb7 fusion failed to activate despite being associated with the Pol II holoenzyme and being directly recruited to the promoter. Interestingly, some acidic activation domains are less effective when the promoter is chromosomally integrated rather than transiently transfected, whereas the Sp1 glutamine-rich activation domain is more effective on integrated reporters. Thus, yeast and mammalian cells differ with respect to transcriptional activation by artificial recruitment of the Pol II holoenzyme.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.