ArticleVisual neuroscience
Differential induction of gene expression by basic fibroblast growth factor and neuroD in cultured retinal pigment epithelial cells.
Article in Visual neuroscience. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Augmented Expression ofIranian journal of public health · 2022Article
- Pigment Epithelia of the Eye: Cell-Type Conversion in Regeneration and Disease.Life (Basel, Switzerland) · 2022Review
- Amniotic fluid promotes the appearance of neural retinal progenitors and neurons in human RPE cell cultures.Molecular vision · 2013Article
- Chick retinal pigment epithelium transdifferentiation assay for proneural activities.Methods in molecular biology (Clifton, N.J.) · 2012Article
- RPE-secreted factors: influence differentiation in human retinal cell line in dose- and density-dependent manner.Journal of ocular biology, diseases, and informatics · 2010Article
- Generating retinal neurons by reprogramming retinal pigment epithelial cells.Expert opinion on biological therapy · 2010Review
- Neurogenin1 effectively reprograms cultured chick retinal pigment epithelial cells to differentiate toward photoreceptors.The Journal of comparative neurology · 2010Article
- Reprogramming retinal pigment epithelium to differentiate toward retinal neurons with Sox2.Stem cells (Dayton, Ohio) · 2009Article
- Reprogramming chick RPE progeny cells to differentiate towards retinal neurons by ash1.Molecular vision · 2008Article
- Exploring RPE as a source of photoreceptors: differentiation and integration of transdifferentiating cells grafted into embryonic chick eyes.Investigative ophthalmology & visual science · 2006Article
- bHLH genes and retinal cell fate specification.Molecular neurobiology · 2005Review
- Enhanced retinal ganglion cell differentiation by ath5 and NSCL1 coexpression.Investigative ophthalmology & visual science · 2004Article
- A role of ath5 in inducing neuroD and the photoreceptor pathway.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Requirement of neuroD for photoreceptor formation in the chick retina.Investigative ophthalmology & visual science · 2004Article
- BETA2/NeuroD1 null mice: a new model for transcription factor-dependent photoreceptor degeneration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- neurogenin2 elicits the genesis of retinal neurons from cultures of nonneural cells.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Atrophy of Müller glia and photoreceptor cells in chick retina misexpressing cNSCL2.Investigative ophthalmology & visual science · 2001Article
- Expression of microtubule-associated protein 2 in benign and malignant melanocytes: implications for differentiation and progression of cutaneous melanoma.The American journal of pathology · 2001Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
Embryonic chick retinal pigment epithelial (RPE) cells can undergo transdifferentiation upon appropriate stimulation. For example, basic fibroblast growth factor (bFGF) induces intact RPE tissue younger than embryonic day 4.5 (E4.5) to transdifferentiate into a neural retina. NeuroD, a gene encoding a basic helix-loop-helix transcription factor, triggers de novo production of cells that resemble young photoreceptor cells morphologically and express general neuron markers (HNK-1/N-CAM and MAP2) and a photoreceptor-specific marker (visinin) from cell cultures of dissociated E6 RPE (Yan & Wang, 1998). The present study examined whether bFGF will lead to the same transdifferentiation phenomenon as neuroD when applied to dissociated, cultured E6 RPE cells, and whether interplay exists between the two factors under the culture conditions. Dissociated E6 RPE cells were cultured in the presence or absence of bFGF, and with or without the addition of retrovirus expressing neuroD. Gene expression was analyzed with immunocytochemistry and in situ hybridization. Unlike neuroD, bFGF did not induce the expression of visinin, or HNK-1/N-CAM and MAP2. However, bFGF elicited the expression of RA4 immunogenicity; yet, many of these RA4-positive cells lacked a neuronal morphology. Addition of bFGF to neuroD-expressing cultures did not alter the number of visinin-expressing cells; misexpression of neuroD in bFGF-treated cultures did not change the number of RA4-positive cells, suggesting the absence of interference or synergistic interaction between the two factors. Our data indicated that bFGF and neuroD induced the expression of different genes in cultured RPE cells.
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