ArticleProceedings of the National Academy of Sciences of the United States of America2000
Cholesterol-dependent clustering of IL-2Ralpha and its colocalization with HLA and CD48 on T lymphoma cells suggest their functional association with lipid rafts.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 151 citations in OpenAlex.
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- Role of C-Terminal Domain and Membrane Potential in the Mobility of Kv1.3 Channels in Immune Synapse Forming T Cells.International journal of molecular sciences · 2022Article
- Obesity and Fatty Acids Promote Mitochondrial Translocation of STAT3 Through ROS-Dependent Mechanisms.Frontiers in aging · 2022Article
- Mechanisms and functions of endocytosis in T cells.Cell communication and signaling : CCS · 2021Review
- Thermogenic Activation Downregulates High Mitophagy Rate in Human Masked and Mature Beige Adipocytes.International journal of molecular sciences · 2020Article
- Metformin Enhances Autophagy and Normalizes Mitochondrial Function to Alleviate Aging-Associated Inflammation.Cell metabolism · 2020Article
- Inhibition of HIV Replication by Apolipoprotein A-I Binding Protein Targeting the Lipid Rafts.mBio · 2020Article
- IL-2 receptors preassemble and signal in the ER/Golgi causing resistance to antiproliferative anti-IL-2Rα therapies.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Dominant role of splenic marginal zone lipid rafts in the classical complement pathway againstCell death discovery · 2019Article
- Interactions between lipids and proteins are critical for organization of plasma membrane-ordered domains in tobacco BY-2 cells.Journal of experimental botany · 2018Article
- Membrane Potential Distinctly Modulates Mobility and Signaling of IL-2 and IL-15 Receptors in T Cells.Biophysical journal · 2018Article
- MHC I Expression Regulates Co-clustering and Mobility of Interleukin-2 and -15 Receptors in T Cells.Biophysical journal · 2016Article
- Annexin A6 regulates interleukin-2-mediated T-cell proliferation.Immunology and cell biology · 2016Article
- Inflammatory and immune markers associated with physical frailty syndrome: findings from Singapore longitudinal aging studies.Oncotarget · 2016Article
- Dynamics of the actin cytoskeleton mediates receptor cross talk: An emerging concept in tuning receptor signaling.The Journal of cell biology · 2016Review
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- MHC class II association with lipid rafts on the antigen presenting cell surface.Biochimica et biophysica acta · 2015Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Immunogold staining and electron microscopy show that IL-2 receptor alpha-subunits exhibit nonrandom surface distribution on human T lymphoma cells. Analysis of interparticle distances reveals that this clustering on the scale of a few hundred nanometers is independent of the presence of IL-2 and of the expression of the IL-2R beta-subunit. Clustering of IL-2Ralpha is confirmed by confocal microscopy, yielding the same average cluster size, approximately 600-800 nm, as electron microscopy. HLA class I and II and CD48 molecules also form clusters of the same size. Disruption of cholesterol-rich lipid rafts with filipin or depletion of membrane cholesterol with methyl-beta-cyclodextrin results in the blurring of cluster boundaries and an apparent dispersion of clusters for all four proteins. Interestingly, the transferrin receptor, which is thought to be located outside lipid rafts, exhibits clusters that are only 300 nm in size and are less affected by modifying the membrane cholesterol content. Furthermore, transferrin receptor clusters hardly colocalize with IL-2Ralpha, HLA, and CD48 molecules (crosscorrelation coefficient is 0.05), whereas IL-2Ralpha colocalizes with both HLA and CD48 (crosscorrelation coefficient is between 0.37 and 0.46). This coclustering is confirmed by electron microscopy. The submicron clusters of IL-2Ralpha chains and their coclustering with HLA and CD48, presumably associated with lipid rafts, could underlie the efficiency of signaling in lymphoid cells.
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