ArticleProceedings of the National Academy of Sciences of the United States of America2000
Structure of a two-domain chitotriosidase from Serratia marcescens at 1.9-A resolution.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 268 citations in OpenAlex.
- Organic matter degradation by oceanic fungi differs between polar and non-polar waters.Nature communications · 2025Article
- Structural and functional significance ofJournal of virology · 2025Article
- Utilizing chitooligosaccharides from shrimp waste biodegradation via recombinant chitinase A: a promising approach for emulsifying hydrocarbon and bioremediation.Microbial cell factories · 2024Article
- Article
- Genomic analysis and chitinase characterization ofFrontiers in microbiology · 2023Article
- Molecular analysis of genes involved in chitin degradation from the chitinolytic bacterium Bacillus velezensis.Antonie van Leeuwenhoek · 2022Article
- Molecular cloning, heterologous expression, and in silico sequence analysis of Enterobacter GH19 class I chitinase (chiRAM gene).Molecular biology reports · 2022Article
- Heterologous Expression of a Thermostable Chitinase fromFoods (Basel, Switzerland) · 2021Article
- Genomic and Proteomic Study ofJournal of proteome research · 2021Article
- Plasmodium chitinases: revisiting a target of transmission-blockade against malaria.Protein science : a publication of the Protein Society · 2021Review
- Recent advances in the bioprospection and applications of chitinolytic bacteria for valorization of waste chitin.Archives of microbiology · 2021Review
- AToxins · 2020Article
- Chitinases ofFrontiers in microbiology · 2019Review
- Chitinase: diversity, limitations, and trends in engineering for suitable applications.Bioscience reports · 2018Review
- Oxygen Activation by Cu LPMOs in Recalcitrant Carbohydrate Polysaccharide Conversion to Monomer Sugars.Chemical reviews · 2018Review
- Systems analysis of the glycoside hydrolase family 18 enzymes fromThe Journal of biological chemistry · 2018Article
- The deduced role of a chitinase containing two nonsynergistic catalytic domains.Acta crystallographica. Section D, Structural biology · 2018Article
- Structure, Catalysis, and Inhibition ofThe Journal of biological chemistry · 2017Article
- Human Chitotriosidase Is an Endo-Processive Enzyme.PloS one · 2017Article
- Ligand-binding specificity and promiscuity of the main lignocellulolytic enzyme families as revealed by active-site architecture analysis.Scientific reports · 2016Article
12 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this paper, we describe the structure of chitinase B from Serratia marcescens, which consists of a catalytic domain with a TIM-barrel fold and a 49-residue C-terminal chitin-binding domain. This chitinase is the first structure of a bacterial exochitinase, and it represents one of only a few examples of a glycosyl hydrolase structure having interacting catalytic and substrate-binding domains. The chitin-binding domain has exposed aromatic residues that contribute to a 55-A long continuous aromatic stretch extending into the active site. Binding of chitin oligomers is blocked beyond the -3 subsite, which explains why the enzyme has chitotriosidase activity and degrades the chitin chain from the nonreducing end. Comparison of the chitinase B structure with that of chitinase A explains why these enzymes act synergistically in the degradation of chitin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.