ArticleJournal of virology2000
Primary murine small intestinal epithelial cells, maintained in long-term culture, are susceptible to rotavirus infection.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Article
- Type I IFN signaling limits hemorrhage-like disease after infection with Japanese encephalitis virus through modulating a prerequisite infection of CD11bJournal of neuroinflammation · 2021Article
- Histo-blood group antigens as divergent factors of groups A and C rotaviruses circulating in humans and different animal species.Emerging microbes & infections · 2020Article
- Lactoferrin translocates to the nucleus of bovine rectal epithelial cells in the presence of Escherichia coli O157:H7.Veterinary research · 2019Article
- Aichi Virus Induces Antiviral Host Defense in Primary Murine Intestinal Epithelial Cells.Viruses · 2019Article
- Modeling Host-Virus Interactions in Viral Infectious Diseases Using Stem-Cell-Derived Systems and CRISPR/Cas9 Technology.Viruses · 2019Review
- Establishment and long-term maintenance of primary intestinal epithelial cells cultured from the rainbow trout,Biology open · 2018Article
- Development and characterization of 2-dimensional culture for buffalo intestinal cells.Cytotechnology · 2018Article
- Triclosan Enhances the Clearing of Pathogenic IntracellularFrontiers in cellular and infection microbiology · 2018Article
- Establishment of immortalized mouse intestinal epithelial cells line and study of effects of Arg-Arg on inflammatory response.In vitro cellular & developmental biology. Animal · 2017Article
- The role of Pygo2 for Wnt/ß-catenin signaling activity during intestinal tumor initiation and progression.Oncotarget · 2016Article
- Article
- Radiation persistently promoted oxidative stress, activated mTOR via PI3K/Akt, and downregulated autophagy pathway in mouse intestine.The international journal of biochemistry & cell biology · 2014Article
- Characteristic and functional analysis of a newly established porcine small intestinal epithelial cell line.PloS one · 2014Article
- Establishment of an in vitro culture system for intestinal epithelial cells from Pheretima aspergillum (E. Perrier).In vitro cellular & developmental biology. Animal · 2014Article
- Small intestinal intraepithelial lymphocytes expressing CD8 and T cell receptor γδ are involved in bacterial clearance during Salmonella enterica serovar Typhimurium infection.Infection and immunity · 2012Article
- Article
- Exposure to heavy ion radiation induces persistent oxidative stress in mouse intestine.PloS one · 2012Article
- TRIF mediates Toll-like receptor 5-induced signaling in intestinal epithelial cells.The Journal of biological chemistry · 2010Article
- Protective role of mouse MBL-C on intestinal mucosa during Shigella flexneri invasion.International immunology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
We describe a method for long-term culture of primary small intestinal epithelial cells (IEC) from suckling mice. IEC were digested from intestinal fragments as small intact units of epithelium (organoids) by using collagenase and dispase. IEC proliferated from organoids on a basement-membrane-coated culture surface and remained viable for 3 weeks. Cultured IEC had the morphologic and functional characteristics of immature enterocytes, notably sustained expression of cytokeratin and alkaline phosphatase. Few mesenchymal cells were present in the IEC cultures. IEC were also cultured from adult BALB/c mice and expressed major histocompatibility complex (MHC) class II antigens for at least 48 h in vitro. Primary IEC supported the growth of rhesus rotavirus (RRV) to a greater extent than a murine small intestinal cell line, m-IC(cl2). Cell-culture-adapted murine rotavirus strain EDIM infected primary IEC and m-IC(cl2) cells to a lesser extent than RRV. Wild-type EDIM did not infect either cell type. Long-term culture of primary murine small intestinal epithelial cells provides a method to study (i) virus-cell interactions, (ii) the capacity of IEC to act as antigen-presenting cells using a wide variety of MHC haplotypes, and (iii) IEC biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.