ArticleJournal of virology2000
Opposite effects of dextran sulfate 500, the polyene antibiotic MS-8209, and Congo red on accumulation of the protease-resistant isoform of PrP in the spleens of mice inoculated intraperitoneally with the scrapie agent.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Neurodegeneration, Mitochondria, and Antibiotics.Metabolites · 2023Review
- Prion therapeutics: Lessons from the past.Prion · 2022Review
- The Role of Vesicle Trafficking Defects in the Pathogenesis of Prion and Prion-Like Disorders.International journal of molecular sciences · 2020Review
- Host prion protein expression levels impact prion tropism for the spleen.PLoS pathogens · 2020Article
- The role of prion strain diversity in the development of successful therapeutic treatments.Progress in molecular biology and translational science · 2020Review
- Prion strain-dependent tropism is maintained between spleen and granuloma and relies on lymphofollicular structures.Scientific reports · 2019Article
- Accelerated, spleen-based titration of variant Creutzfeldt-Jakob disease infectivity in transgenic mice expressing human prion protein with sensitivity comparable to that of survival time bioassay.Journal of virology · 2014Article
- Article
- Targeting of prion-infected lymphoid cells to the central nervous system accelerates prion infection.Journal of neuroinflammation · 2012Article
- Oligomeric-induced activity by thienyl pyrimidine compounds traps prion infectivity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Glypican-1 mediates both prion protein lipid raft association and disease isoform formation.PLoS pathogens · 2009Article
- Chemically induced accumulation of GAGs delays PrP(Sc) clearance but prolongs prion disease incubation time.Cellular and molecular neurobiology · 2008Article
- Antihypertensive drug guanabenz is active in vivo against both yeast and mammalian prions.PloS one · 2008Article
- Prominent and persistent extraneural infection in human PrP transgenic mice infected with variant CJD.PloS one · 2008Article
- Prion strain- and species-dependent effects of antiprion molecules in primary neuronal cultures.Journal of virology · 2007Article
- Mechanistic insights into the cure of prion disease by novel antiprion compounds.Journal of virology · 2007Article
- Fatal neurological disease in scrapie-infected mice induced for experimental autoimmune encephalomyelitis.Journal of virology · 2007Article
- Early and rapid engraftment of bone marrow-derived microglia in scrapie.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Potent antiscrapie activities of degenerate phosphorothioate oligonucleotides.Antimicrobial agents and chemotherapy · 2006Article
- Sulfated glycans and elevated temperature stimulate PrP(Sc)-dependent cell-free formation of protease-resistant prion protein.The EMBO journal · 2001Article
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Authors and funding
7 authors.
Funding
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Abstract
The mode and the site of action of the major antiscrapie drugs have been studied by investigating their effects on the abnormal protease-resistant isoform of PrP (PrPres) and on its accumulation in mouse spleen. Day-by-day PrPres accumulation in the spleen and in other peripheral organs was first monitored to describe the early steps of scrapie pathogenesis. Three phases were identified: the detection of scrapie inoculum on the day of scrapie infection, a clearance phase, and then the peripheral accumulation of PrPres. In a second step, the effects of the polyene antibiotic MS-8209, the polyanion dextran sulfate 500 (DS500), and Congo red were assessed on these phases, after the drugs were coincubated with scrapie inoculum. Highly different mechanisms and sites of action were apparent. MS-8209 had a weak effect on the accumulation of PrPres in spleen, suggesting another site of intervention for this drug. DS500 delayed the beginning of the clearance phase but then blocked PrPres synthesis for a long period of time, probably because of its immunological effects on the spleen. Surprisingly, Congo red suppressed the clearance phase of scrapie inoculum and then increased transiently accumulation of PrPres in spleen. We showed in vitro that this effect was related to a direct enhancement of the protease resistance of PrPres by the drug.
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