ArticleJournal of virology2000
Rubella virus nonstructural protein protease domains involved in trans- and cis-cleavage activities.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Identification and classification of papain-like cysteine proteinases.The Journal of biological chemistry · 2023Article
- Crystal structure of the Rubella virus protease reveals a unique papain-like protease fold.The Journal of biological chemistry · 2022Article
- Exogenous Rubella Virus Capsid Proteins Enhance Virus Genome Replication.Pathogens (Basel, Switzerland) · 2022Article
- Molecular and Structural Insights into the Life Cycle of Rubella Virus.Journal of virology · 2021Review
- Molecular Biology and Infection of Hepatitis E Virus.Frontiers in microbiology · 2016Review
- Analysis of whole genome sequences of 16 strains of rubella virus from the United States, 1961-2009.Virology journal · 2013Article
- Determinants in the maturation of rubella virus p200 replicase polyprotein precursor.Journal of virology · 2012Article
- Macromolecular assembly-driven processing of the 2/3 cleavage site in the alphavirus replicase polyprotein.Journal of virology · 2012Article
- Interaction of Sindbis virus non-structural protein 3 with poly(ADP-ribose) polymerase 1 in neuronal cells.The Journal of general virology · 2009Article
- The nsP3 macro domain is important for Sindbis virus replication in neurons and neurovirulence in mice.Virology · 2009Article
- Identification of a Ca2+-binding domain in the rubella virus nonstructural protease.Journal of virology · 2007Article
- The rubella virus nonstructural protease recognizes itself via an internal sequence present upstream of the cleavage site for trans-activity.Archives of virology · 2006Article
- Rubella virus capsid protein modulates viral genome replication and virus infectivity.Journal of virology · 2004Article
- Complementation of a deletion in the rubella virus p150 nonstructural protein by the viral capsid protein.Journal of virology · 2003Article
- Mutational analysis of the rubella virus nonstructural polyprotein and its cleavage products in virus replication and RNA synthesis.Journal of virology · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rubella virus (RV) genomic RNA contains two large open reading frames (ORFs): a 5'-proximal ORF encoding nonstructural proteins (NSPs) that function primarily in viral RNA replication and a 3'-proximal ORF encoding the viral structural proteins. Proteolytic processing of the RV NSP ORF translation product p200 is essential for viral replication. Processing of p200 to two mature products (p150 and p90) in the order NH(2)-p150-p90-COOH is carried out by an RV-encoded protease residing in the C-terminal region of p150. The RV nonstructural protease (NS-pro) belongs to a viral papain-like protease family that cleaves the polyprotein both in trans and in cis. A conserved X domain of unknown function was found from previous sequence analysis to be associated with NS-pro. To define the domains responsible for cis- and trans-cleavage activities and the function of the X domain in terms of protease activity, an in vitro translation system was employed. We demonstrated that the NSP region from residue 920 to 1296 is necessary for trans-cleavage activity. The domain from residue 920 to 1020 is not required for cis-cleavage activity. The X domain located between residues 834 and 940, outside the regions responsible for both cis- and trans-cleavage activities of NS-pro, was found to be important for NS-pro trans-cleavage activity but not for cis-cleavage activity. Analysis of sequence homology and secondary structure of the RV NS-pro catalytic region reveals a folding structure similar to that of papain.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.