ArticleGenes & development2000
H19 and Igf2 monoallelic expression is regulated in two distinct ways by a shared cis acting regulatory region upstream of H19.
Article in Genes & development, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 141 citations in OpenAlex.
- Sperm-carried IGF2: towards the discovery of a spark contributing to embryo growth and development.Molecular human reproduction · 2024Article
- Five nucleotides found in RCTG motifs are essential for post-fertilization methylation imprinting of the H19 ICR in YAC transgenic mice.Nucleic acids research · 2023Article
- The imprinted Igf2-Igf2r axis is critical for matching placental microvasculature expansion to fetal growth.Developmental cell · 2022Article
- Loss of imprinting of the Igf2-H19 ICR1 enhances placental endocrine capacity via sex-specific alterations in signalling pathways in the mouse.Development (Cambridge, England) · 2022Article
- Orientation of mouse H19 ICR affects imprinted H19 gene expression through promoter methylation-dependent and -independent mechanisms.Communications biology · 2021Article
- Cardiac pathologies in mouse loss of imprinting models are due to misexpression of H19 long noncoding RNA.eLife · 2021Article
- Mesenchyme-derived IGF2 is a major paracrine regulator of pancreatic growth and function.PLoS genetics · 2020Article
- Modeling human epigenetic disorders in mice: Beckwith-Wiedemann syndrome and Silver-Russell syndrome.Disease models & mechanisms · 2020Review
- The Good, the Bad, the Question-Cancers · 2020Review
- Recapitulation of gametic DNA methylation and its post-fertilization maintenance with reassembled DNA elements at the mouse Igf2/H19 locus.Epigenetics & chromatin · 2020Article
- Long Noncoding RNA Discovery in Cardiovascular Disease: Decoding Form to Function.Circulation research · 2018Review
- Loss of imprinting mutations define both distinct and overlapping roles for misexpression of IGF2 and of H19 lncRNA.Nucleic acids research · 2017Article
- CRISPR Cas9-guided chromatin immunoprecipitation identifies miR483 as an epigenetic modulator of IGF2 imprinting in tumors.Oncotarget · 2017Article
- The Interplay of LncRNA-H19 and Its Binding Partners in Physiological Process and Gastric Carcinogenesis.International journal of molecular sciences · 2017Review
- Article
- H19ICR mediated transcriptional silencing does not require target promoter methylation.Biochemical and biophysical research communications · 2016Article
- TRIM28 Controls Genomic Imprinting through Distinct Mechanisms during and after Early Genome-wide Reprogramming.Cell reports · 2015Article
- Influence of a CTCF-Dependent Insulator on Multiple Aspects of Enhancer-Mediated Chromatin Organization.Molecular and cellular biology · 2015Article
- Regulation of tumor cell migration and invasion by the H19/let-7 axis is antagonized by metformin-induced DNA methylation.Oncogene · 2015Article
- Promoter histone H3K27 methylation in the control of IGF2 imprinting in human tumor cell lines.Human molecular genetics · 2014Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
H19 and Igf2 are expressed in a monoallelic fashion from the maternal and paternal chromosomes, respectively. A region upstream of H19 has been shown to regulate such imprinted expression of both genes in cis. We have taken advantage of a loxP/cre recombinase-based strategy to delete this region in mice in a conditional manner to determine the temporal requirement of the upstream region in initiating and maintaining the imprinted expression of H19 and Igf2. Analysis of allele-specific expression of H19 and Igf2 and DNA methylation at the H19 promoter demonstrates that this region controls the monoallelic expression of the two genes in different ways, suggesting that it harbors two functionally distinct regulatory elements. Continued presence of the region is required to silence maternal Igf2 in accordance with its proposed role as an insulator. However, it does not have a direct role in keeping the paternal H19 promoter silenced. Instead, on the paternal chromosome, the upstream element mediates epigenetic modifications of the H19 promoter region during development, leading to transcriptional silencing of H19. Thereafter, its presence is redundant for preventing transcription. Presently, this temporal requirement of the silencing element appears to be a unique cis activity in the mammalian system. However, it is likely that other cis-acting elements, positive and negative, have the ability to effect stable changes in the chromatin structure and are not constantly required to give signals to the transcriptional machinery.
Indexed as
Identifiers
10817754PMC316622W2159250091What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.