ArticleInfection and immunity2000
An OmpA-like protein from Acinetobacter spp. stimulates gastrin and interleukin-8 promoters.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- The immune microenvironment in gastric adenocarcinoma.Nature reviews. Gastroenterology & hepatology · 2022Review
- Article
- Impact of Helicobacter pylori eradication on the gastric microbiome.Gut pathogens · 2021Article
- Stomach gastrin is regulated by sodium via PPAR-α and dopamine D1 receptor.Journal of molecular endocrinology · 2020Article
- Global Dynamic Proteome Study of a Pellicle-forming Acinetobacter baumannii Strain.Molecular & cellular proteomics : MCP · 2017Article
- Comparative analysis of surface-exposed virulence factors of Acinetobacter baumannii.BMC genomics · 2014Article
- H-NS plays a role in expression of Acinetobacter baumannii virulence features.Infection and immunity · 2013Article
- Bacterial flora concurrent with Helicobacter pylori in the stomach of patients with upper gastrointestinal diseases.World journal of gastroenterology · 2012Article
- Article
- Porin isolated from the outer membrane of Erwinia amylovora and its encoding gene.Current microbiology · 2007Article
- Article
- Channel formation by CarO, the carbapenem resistance-associated outer membrane protein of Acinetobacter baumannii.Antimicrobial agents and chemotherapy · 2005Article
- Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium.Nucleic acids research · 2004Article
- Phospholipase A2 group IIA expression in gastric adenocarcinoma is associated with prolonged survival and less frequent metastasis.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Gastritis and hypergastrinemia due to Acinetobacter lwoffii in mice.Infection and immunity · 2002Article
- The active component of the bioemulsifier alasan from Acinetobacter radioresistens KA53 is an OmpA-like protein.Journal of bacteriology · 2002Article
- Moraxella catarrhalis: from emerging to established pathogen.Clinical microbiology reviews · 2002Review
- Vibrio fischeri outer membrane protein OmpU plays a role in normal symbiotic colonization.Journal of bacteriology · 2001Article
- Emulsifying activities of purified Alasan proteins from Acinetobacter radioresistens KA53.Applied and environmental microbiology · 2001Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Bacterial overgrowth in the stomach may occur under conditions of diminished or absent acid secretion. Under these conditions, secretion of the hormone gastrin is elevated. Alternatively, bacterial factors may directly stimulate gastrin. Consistent with this hypothesis, we found that mice colonized for 2 months with a mixed bacterial culture of opportunistic pathogens showed an increase in serum gastrin. To examine regulation of gene expression by bacterial proteins, stable transformants of AGS cells expressing gastrin or interleukin-8 (IL-8) promoters were cocultured with live organisms. Both whole-cell sonicates and a heat-stable fraction were also coincubated with the cells. A level of 10(8) organisms per ml stimulated both the gastrin and IL-8 promoters. Heat-stable proteins prepared from these bacterial sonicates stimulated the promoter significantly more than the live organism or unheated sonicates. A 38-kDa heat-stable protein stimulating the gastrin and IL-8 promoters was cloned and found to be an OmpA-related protein. Immunoblotting using antibody to the OmpA-like protein identified an Acinetobacter sp. as the bacterial species that expressed this protein and colonized the mouse stomach. Moreover, reintubation of mice with a pure culture of the Acinetobacter sp. caused gastritis. We conclude that bacterial colonization of the stomach may increase serum gastrin levels in part through the ability of the bacteria to produce OmpA-like proteins that directly stimulate gastrin and IL-8 gene expression. These results implicate OmpA-secreting bacteria in the activation of gastrin gene expression and raise the possibility that a variety of organisms may contribute to the increase in serum gastrin and subsequent epithelial cell proliferation in the hypochlorhydric stomach.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.