ArticleInfection and immunity2000
Shigella flexneri IpaH(7.8) facilitates escape of virulent bacteria from the endocytic vacuoles of mouse and human macrophages.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 101 citations in OpenAlex.
- Virulence factors associated with pediatric shigellosis in Brazilian Amazon.BioMed research international · 2014Trial
- Syndromic cholera diagnosis masks diverse causes of diarrhoeal disease in Burundi revealed by portable metagenomics.PLoS neglected tropical diseases · 2026Article
- ThebioRxiv : the preprint server for biology · 2026Article
- Microtubules provide force to promote membrane uncoating in vacuolar escape for a cyto-invasive bacterial pathogen.Nature communications · 2024Article
- New insights into the putative role of leucine-rich repeat proteins ofFrontiers in cellular and infection microbiology · 2024Review
- The NEL Family of Bacterial E3 Ubiquitin Ligases.International journal of molecular sciences · 2022Review
- When the Phagosome Gets Leaky: Pore-Forming Toxin-Induced Non-Canonical Autophagy (PINCA).Frontiers in cellular and infection microbiology · 2022Article
- Functional degradation: A mechanism of NLRP1 inflammasome activation by diverse pathogen enzymes.Science (New York, N.Y.) · 2019Article
- Recent insights into Shigella.Current opinion in infectious diseases · 2018Review
- Bacterium-Derived Cell-Penetrating Peptides Deliver Gentamicin To Kill Intracellular Pathogens.Antimicrobial agents and chemotherapy · 2017Article
- How Do the Virulence Factors ofFrontiers in cellular and infection microbiology · 2017Review
- Purine Biosynthesis Metabolically Constrains Intracellular Survival of Uropathogenic Escherichia coli.Infection and immunity · 2017Article
- Characterization of a multicomponent live, attenuated Shigella flexneri vaccine.Pathogens and disease · 2016Article
- The genomic signatures of Shigella evolution, adaptation and geographical spread.Nature reviews. Microbiology · 2016Review
- Bacteria-host relationship: ubiquitin ligases as weapons of invasion.Cell research · 2016Review
- Implications of Spatiotemporal Regulation of Shigella flexneri Type Three Secretion Activity on Effector Functions: Think Globally, Act Locally.Frontiers in cellular and infection microbiology · 2016Review
- Cytosolic Access of Intracellular Bacterial Pathogens: The Shigella Paradigm.Frontiers in cellular and infection microbiology · 2016Review
- Macrophage cell death upon intracellular bacterial infection.Macrophage · 2015Article
- The Rising Dominance of Shigella sonnei: An Intercontinental Shift in the Etiology of Bacillary Dysentery.PLoS neglected tropical diseases · 2015Review
- Shigella IpaH7.8 E3 ubiquitin ligase targets glomulin and activates inflammasomes to demolish macrophages.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The behavior of Shigella flexneri ipaH mutants was studied in human monocyte-derived macrophages (HMDM), in 1-day-old human monocytes, and in J774 mouse macrophage cell line. In HMDM, strain pWR700, an ipaH(7.8) deletion mutant of S. flexneri 2a strain 2457T, behaved like the wild-type strain 2457T. This strain caused rapid host cell death by oncosis, and few bacterial CFU were recovered after incubation in the presence of gentamicin as previously described for 2457T-infected HMDM. However, analysis of bacterial compartmentalization within endocytic vacuoles with gentamicin and chloroquine indicated that more pWR700 than 2457T was present within the endocytic vacuoles of HMDM, suggesting that ipaH(7.8) deletion mutant transited more slowly from the vacuoles to the cytoplasm. In contrast to findings with HMDM, CFU recovered from pWR700-infected mouse J774 cells were 2 to 3 logs higher than CFU from 2457T-infected J774 cells. These values exceeded CFU recovered after infection of J774 cells with plasmid-cured avirulent strain M4243A1. Incubation with gentamicin and chloroquine clearly showed that pWR700 within J774 cells was mostly present within the endocytic vacuoles. This distribution pattern was similar to that seen with M4243A1 and contrasted with the pattern seen with 2457T. Complementation of pWR700 with a recombinant clone expressing ipaH(7. 8) restored the intracellular distribution of bacteria to that seen with the wild-type strain. Strains with deletions in ipaH(4.5) or ipaH(9.8), however, behaved like 2457T in both HMDM and J774 cells. The distribution profile of pWR700 in 1-day-old monocytes was similar to that seen in J774 cells. Like infected J774 cells, 1-day-old human monocytes demonstrated apoptosis upon infection with virulent Shigella. These results suggest that a role of the ipaH(7. 8) gene product is to facilitate the escape of the virulent bacteria from the phagocytic vacuole of monocytes and macrophages.
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