ArticleInfection and immunity2000
Identification of potential vaccine and drug target candidates by expressed sequence tag analysis and immunoscreening of Onchocerca volvulus larval cDNA libraries.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 96 citations in OpenAlex.
- Chromosome-ContiguousInternational journal of molecular sciences · 2025Article
- Crystal structure of an extracellular superoxide dismutase from Onchocerca volvulus and implications for parasite-specific drug development.Acta crystallographica. Section F, Structural biology communications · 2022Article
- Advancing a Human Onchocerciasis Vaccine From Antigen Discovery to Efficacy Studies Against Natural Infection of Cattle WithFrontiers in cellular and infection microbiology · 2022Review
- Expression of Ascaris lumbricoides putative virulence-associated genes when infecting a human host.Parasites & vectors · 2021Article
- Linear epitopes in Onchocerca volvulus vaccine candidate proteins and excretory-secretory proteins.Parasite immunology · 2018Article
- Onchocerca volvulus: The Road from Basic Biology to a Vaccine.Trends in parasitology · 2018Review
- The genome of Onchocerca volvulus, agent of river blindness.Nature microbiology · 2016Article
- Vaccination of Gerbils with Bm-103 and Bm-RAL-2 Concurrently or as a Fusion Protein Confers Consistent and Improved Protection against Brugia malayi Infection.PLoS neglected tropical diseases · 2016Article
- Exploiting the Polypharmacology of ß-Carbolines to Disrupt O. volvulus Molting.ACS medicinal chemistry letters · 2015Article
- Transcriptome sequencing and analysis of the zoonotic parasite Spirometra erinacei spargana (plerocercoids).Parasites & vectors · 2014Article
- Anti-Onchocerca activity and phytochemical analysis of an essential oil from Cyperus articulatus L.BMC complementary and alternative medicine · 2014Article
- Hc-daf-2 encodes an insulin-like receptor kinase in the barber's pole worm, Haemonchus contortus, and restores partial dauer regulation.International journal for parasitology · 2014Article
- Nematode-derived proteins suppress proliferation and cytokine production of antigen-specific T cells via induction of cell death.PloS one · 2013Article
- Screening the Schistosoma mansoni transcriptome for genes differentially expressed in the schistosomulum stage in search for vaccine candidates.Parasitology research · 2011Article
- The Onchocerca volvulus cysteine proteinase inhibitor, Ov-CPI-2, is a target of protective antibody response that increases with age.PLoS neglected tropical diseases · 2010Article
- Screening of different classes of proteases in microfilarial and adult stages of Setaria cervi.Parasitology research · 2009Article
- Systematic analysis of insertions and deletions specific to nematode proteins and their proposed functional and evolutionary relevance.BMC evolutionary biology · 2009Article
- Identification and cloning of a novel tetraspanin (TSP) homologue from Brugia malayi.DNA sequence : the journal of DNA sequencing and mapping · 2008Article
- Molecular cloning, purification and characterisation of myosin of human lymphatic filarial parasite Brugia malayi.Parasitology research · 2008Article
- The bacterial second messenger cdiGMP exhibits promising activity as a mucosal adjuvant.Clinical and vaccine immunology : CVI · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
The search for appropriate vaccine candidates and drug targets against onchocerciasis has so far been confronted with several limitations due to the unavailability of biological material, appropriate molecular resources, and knowledge of the parasite biology. To identify targets for vaccine or chemotherapy development we have undertaken two approaches. First, cDNA expression libraries were constructed from life cycle stages that are critical for establishment of Onchocerca volvulus infection, the third-stage larvae (L3) and the molting L3. A gene discovery effort was then initiated by random expressed sequence tag analysis of 5,506 cDNA clones. Cluster analyses showed that many of the transcripts were up-regulated and/or stage specific in either one or both of the cDNA libraries when compared to the microfilariae, L2, and both adult stages of the parasite. Homology searches against the GenBank database facilitated the identification of several genes of interest, such as proteinases, proteinase inhibitors, antioxidant or detoxification enzymes, and neurotransmitter receptors, as well as structural and housekeeping genes. Other O. volvulus genes showed homology only to predicted genes from the free-living nematode Caenorhabditis elegans or were entirely novel. Some of the novel proteins contain potential secretory leaders. Secondly, by immunoscreening the molting L3 cDNA library with a pool of human sera from putatively immune individuals, we identified six novel immunogenic proteins that otherwise would not have been identified as potential vaccinogens using the gene discovery effort. This study lays a solid foundation for a better understanding of the biology of O. volvulus as well as for the identification of novel targets for filaricidal agents and/or vaccines against onchocerciasis based on immunological and rational hypothesis-driven research.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.