ArticleInfection and immunity2000
Role of hyphal formation in interactions of Candida albicans with endothelial cells.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed, 181 citations in OpenAlex.
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- RFX transcription factor in the human-associated yeast Candida albicans regulates adhesion to oral epithelium.Molecular microbiology · 2024Article
- Molecular mechanisms that govern infection and antifungal resistance in Mucorales.Microbiology and molecular biology reviews : MMBR · 2024Review
- A mouse model of immunosuppression facilitates oralFrontiers in cellular and infection microbiology · 2024Article
- Clotrimazole-Loaded Borneol-Based In Situ Forming Gel as Oral Sprays for Oropharyngeal Candidiasis Therapy.Gels (Basel, Switzerland) · 2023Article
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- Significance of Pulmonary Endothelial Injury and the Role of Cyclooxygenase-2 and Prostanoid Signaling.Bioengineering (Basel, Switzerland) · 2023Review
- Serum bridging molecules drive candidal invasion of human but not mouse endothelial cells.PLoS pathogens · 2022Article
- Candidalysin delivery to the invasion pocket is critical for host epithelial damage induced by Candida albicans.Cellular microbiology · 2021Article
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- Immune Sensing ofJournal of fungi (Basel, Switzerland) · 2021Review
- Efg1 and Cas5 Orchestrate Cell Wall Damage Response to Caspofungin in Candida albicans.Antimicrobial agents and chemotherapy · 2021Article
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- Oral Candidiasis: A Disease of Opportunity.Journal of fungi (Basel, Switzerland) · 2020Review
- Mannan detecting C-type lectin receptor probes recognise immune epitopes with diverse chemical, spatial and phylogenetic heterogeneity in fungal cell walls.PLoS pathogens · 2020Article
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- Exposure of Candida albicans β (1,3)-glucan is promoted by activation of the Cek1 pathway.PLoS genetics · 2019Article
- Yeast and Filaments Have Specialized, Independent Activities in a Zebrafish Model of Candida albicans Infection.Infection and immunity · 2018Article
- Rapid Phenotypic and Genotypic Diversification After Exposure to the Oral Host Niche inGenetics · 2018Article
25 more citing papers are in PubMed but not listed here.
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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The ability to change from yeast to hyphal morphology is a major virulence determinant of Candida albicans. Mutants with defined defects in filamentation regulatory pathways have reduced virulence in mice. However, is it poorly understood why hyphal formation is critical for C. albicans to cause hematogenously disseminated infections. We used recently constructed mutants to examine the role of hyphal formation in the interactions of C. albicans with endothelial cells in vitro. These interactions included the ability of the mutants to invade and injure endothelial cells. Because the formation of hyphae may influence the host inflammatory response to C. albicans, we also investigated the capacity of these mutants to stimulate endothelial cells to express E-selectin and intercellular adhesion molecule 1. We infected endothelial cells with C. albicans strains containing homozygous null mutations in the following filamentation regulatory genes: CLA4, CPH1, EFG1, and TUP1. Whereas the wild-type strain formed true hyphae on endothelial cells, we found that neither the Deltaefg1 nor the Deltacph1 Deltaefg1 double mutant germinated. The Deltatup1 mutant formed only pseudohyphae. We also found that the Deltaefg1, Deltacph1 Deltaefg1, and Deltatup1 mutants had significantly reduced capacities to invade and injure endothelial cells. Therefore, Efg1p and Tup1p contribute to virulence by regulating hyphal formation and the factors that enable C. albicans to invade and injure endothelial cells. With the exception of the Deltacph1 Deltaefg1 mutant, all other mutants stimulated endothelial cells to express at least one of the leukocyte adhesion molecules. Therefore, the combined activities of Cph1p and Efg1p are required for C. albicans to stimulate a proinflammatory response in endothelial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.