ArticleInfection and immunity2000
Immune response against a cross-reactive epitope on the heat shock protein 60 homologue of Helicobacter pylori.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Is Helicobacter pylori infection associated with Henoch-Schonlein purpura in Chinese children? a meta-analysis.World journal of pediatrics : WJP · 2012Pooled it
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- Recombinant L. lactis vaccine LL-plSAM-WAE targeting four virulence factors provides mucosal immunity against H. pylori infection.Microbial cell factories · 2024Article
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- Causal associations of circulating Helicobacter pylori antibodies with stroke and the mediating role of inflammation.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2023Article
- Mechanistic Insight Into the Interaction BetweenFrontiers in microbiology · 2019Article
- Oral Immunization with a Multivalent Epitope-Based Vaccine, Based on NAP, Urease, HSP60, and HpaA, Provides Therapeutic Effect onFrontiers in cellular and infection microbiology · 2017Article
- Current views of the relationship between Helicobacter pylori and Henoch-Schonlein purpura in children.World journal of clinical pediatrics · 2016Review
- B-cell epitopes in GroEL of Francisella tularensis.PloS one · 2014Article
- Immunoproteomics: the key to discovery of new vaccine antigens against bacterial respiratory infections.Current protein & peptide science · 2012Review
- Comparison of epitope specificity of anti-heat shock protein 60/65 IgG type antibodies in the sera of healthy subjects, patients with coronary heart disease and inflammatory bowel disease.Cell stress & chaperones · 2012Article
- Autoimmunity, infectious immunity, and atherosclerosis.Journal of clinical immunology · 2009Review
- Serum antibodies to Porphyromonas gingivalis chaperone HtpG predict health in periodontitis susceptible patients.PloS one · 2008Article
- Long-term infection of Mongolian gerbils with Helicobacter pylori: microbiological, histopathological, and serological analyses.Clinical and diagnostic laboratory immunology · 2005Article
- Article
- Expression of Helicobacter pylori Hsp60 protein and its immunogenicity.World journal of gastroenterology · 2003Article
- Effect of bacterial flora on postimmunization gastritis following oral vaccination of mice with Helicobacter pylori heat shock protein 60.Clinical and diagnostic laboratory immunology · 2003Article
- B-Cell epitope mapping of the VapA protein of Rhodococcus equi: implications for early detection of R. equi disease in foals.Journal of clinical microbiology · 2001Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously established a monoclonal antibody (MAb), designated H9, which reacts with the heat shock protein 60 (HSP60) homologue of Helicobacter pylori as well as with other bacterial and human HSP60s. To determine the importance of a cross-reactive epitope on H. pylori HSP60 in H. pylori immunopathogenesis, we performed (i) mapping of an epitope on H. pylori HSP60 recognized by the H9 MAb, (ii) analysis of immunoglobulin G responses of patients with or without H. pylori infection to its epitope region, and (iii) studies of the protective effect of immunization with its epitope region on H. pylori infection in mice. The epitope recognized by the H9 MAb was mapped to the sequence of amino acids 189 to 203 (VEGMQFDRGYLSPYF) on the H. pylori HSP60 molecule. It was confirmed that the synthesized peptide designated pH9 was recognized by the H9 MAb. Enzyme-linked immunosorbent assay analysis showed that patients with H. pylori infection (n = 349) had significantly lower titers of pH9 antibody than did uninfected patients (n = 200) (P < 0.001), but this was not the case with purified H. pylori HSP60 recombinant Escherichia coli GroEL, or recombinant human HSP60. In C57BL/6 mice immunized with the pH9 peptide with Freund's complete adjuvant (FCA), the number of H. pylori organisms colonizing the stomach was significantly lower than that in mice immunized with pCont plus FCA (P < 0.0001) or FCA only (P < 0.005). The results suggest that the immune response to the cross-reactive epitope (pH9 region) on H. pylori HSP60 is unique and might be associated with protection against H. pylori infection.
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