ArticleInfection and immunity2000
Schistosoma mansoni phosphoenolpyruvate carboxykinase, a novel egg antigen: immunological properties of the recombinant protein and identification of a T-cell epitope.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Identification and characterization of the elusive protein backbone of the immuno-dominant and species-specific Em2(G11) metacestode antigen ofFrontiers in parasitology · 2025Article
- Proteomic Analysis of Extracellular Vesicles FromFrontiers in cellular and infection microbiology · 2022Article
- Modulation of the Host Immune Response by Schistosome Egg-Secreted Proteins Is a Critical Avenue of Host-Parasite Communication.Pathogens (Basel, Switzerland) · 2021Review
- A comparative proteomics analysis of the egg secretions of three major schistosome species.Molecular and biochemical parasitology · 2020Article
- Article
- Alteration of immunoproteome profile of Echinococcus granulosus hydatid fluid with progression of cystic echinococcosis.Parasites & vectors · 2015Article
- Transcriptome profiles of the protoscoleces of Echinococcus granulosus reveal that excretory-secretory products are essential to metabolic adaptation.PLoS neglected tropical diseases · 2014Article
- Induction and regulation of pathogenic Th17 cell responses in schistosomiasis.Seminars in immunopathology · 2012Review
- T regulatory cell responses to immunization with a soluble egg antigen in Schistosoma mansoni-infected mice.The Korean journal of parasitology · 2012Article
- Identification of cytokeratin 18 as a biomarker of mouse and human hepatosplenic schistosomiasis.Infection and immunity · 2011Article
- Differential patterns of liver proteins in experimental murine hepatosplenic schistosomiasis.Infection and immunity · 2010Article
- Leucine aminopeptidase is an immunodominant antigen of Fasciola hepatica excretory and secretory products in human infections.Clinical and vaccine immunology : CVI · 2008Article
- Fasciola hepatica: identification of CD4+ T-helper epitopes from the 11.5 kDa saposin-like protein SAP-2 using synthetic peptides.Experimental parasitology · 2007Article
- Regulation of granulomatous inflammation in experimental models of schistosomiasis.Infection and immunity · 2004Review
- Identification of a novel T-cell epitope in soluble egg antigen of Schistosoma japonicum.Infection and immunity · 2001Article
- Schistosome infection stimulates host CD4(+) T helper cell and B-cell responses against a novel egg antigen, thioredoxin peroxidase.Infection and immunity · 2001Article
- A proteomic approach to identify proteins from Trichuris trichiura extract with immunomodulatory effects.Parasite immunologyArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
In schistosomiasis mansoni, hepatic granulomatous inflammation surrounding parasite eggs is mediated by CD4(+) T helper (Th) cells sensitized to schistosomal egg antigens (SEA). We previously showed that a prominent lymphoproliferative response of CD4(+) Th cells from schistosome-infected C57BL/6 (BL/6) mice was directed against a 62-kDa component of SEA. A partial amino acid sequence of the 62-kDa component was found to be identical with one present in the enzyme phosphoenolpyruvate carboxykinase (PEPCK). Based on this sequence, a cDNA clone containing the entire coding region of PEPCK was identified, and the full recombinant Schistosoma mansoni PEPCK (rSm-PEPCK) of 626 amino acids was purified from a prokaryotic expression system. rSm-PEPCK strongly stimulated a specific T-cell hybridoma, 4E6, as well as CD4(+) Th cells from SEA-immunized BL/6 mice and from infected BL/6, CBA, and BALB/c mice. In the infected mice, rSm-PEPCK elicited significant gamma interferon production as well as, to a lesser extent, production of interleukin-2 and -5. In BL/6 and BALB/c mice, the CD4(+) Th cell response to rSm-PEPCK was greater than that directed against the egg antigen Sm-p40; conversely, CBA mice responded better to Sm-p40 than to Sm-PEPCK. A 12-amino-acid region (residues 398 to 409: DKSKDPKAHPNS) was demonstrated to contain a T-cell epitope; synthetic peptides containing this epitope significantly stimulated specific hybridoma 4E6 and polyclonal CD4(+) Th cells. The identification and characterization of immunogenic egg components will contribute to the understanding and possible control of T-cell-mediated schistosomal disease.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.