ArticleInfection and immunity2000
Adhesion of Aspergillus species to extracellular matrix proteins: evidence for involvement of negatively charged carbohydrates on the conidial surface.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 98 citations in OpenAlex.
- Pathogenicity and virulence ofVirulence · 2023Review
- Fibronectin-binding molecules of Scedosporium apiospermum: focus on adhesive events.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Exposure ofJournal of fungi (Basel, Switzerland) · 2023Article
- Protective role of CFTR during fungal infection of cystic fibrosis bronchial epithelial cells withFrontiers in cellular and infection microbiology · 2023Article
- Review
- Growth ofJournal of fungi (Basel, Switzerland) · 2022Review
- Bio-control on the contamination of Ochratoxin A in food: Current research and future prospects.Current research in food science · 2022Review
- Fungi-A Component of the Oral Microbiome Involved in Periodontal Diseases.Advances in experimental medicine and biology · 2022Article
- Review
- Identifying candidate Aspergillus pathogenicity factors by annotation frequency.BMC microbiology · 2020Article
- Distinct Binding Interactions of αBiophysical journal · 2019Article
- It takes a village: Phagocytes play a central role in fungal immunity.Seminars in cell & developmental biology · 2019Review
- Human Bronchial Epithelial Cells Inhibit Aspergillus fumigatus Germination of Extracellular Conidia via FleA Recognition.Scientific reports · 2018Article
- Lung colonization by Aspergillus fumigatus is controlled by ZNF77.Nature communications · 2018Article
- Calcium sequestration by fungal melanin inhibits calcium-calmodulin signalling to prevent LC3-associated phagocytosis.Nature microbiology · 2018Article
- Anti-Aspergillus Activities of the Respiratory Epithelium in Health and Disease.Journal of fungi (Basel, Switzerland) · 2018Review
- Article
- Comparative Analysis of Inflammatory Cytokine Release and Alveolar Epithelial Barrier Invasion in a TranswellFrontiers in microbiology · 2018Article
- Clinical significance of Aspergillus species isolated from respiratory specimens in patients with Mycobacterium avium complex lung disease.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
- Aspergillus fumigatus morphology and dynamic host interactions.Nature reviews. Microbiology · 2017Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Invasive lung disease caused by Aspergillus species is a potentially fatal infection in immunocompromised patients. The adhesion of Aspergillus fumigatus conidia to proteins in the basal lamina is thought to be an initial step in the development of invasive aspergillosis. The purpose of this study was to determine the mechanism of adhesion of A. fumigatus conidiospores to basal-lamina proteins and to determine whether conidia possess unique adhesins which allow them to colonize the host. We compared conidia from different Aspergillus species for the ability to bind to purified fibronectin and intact basal lamina. Adhesion assays using immobilized fibronectin or type II pneumocyte-derived basal lamina showed that A. fumigatus conidia bound significantly better than those of other Aspergillus species to both fibronectin and intact basal lamina. Neither desialylation nor complete deglycosylation of fibronectin decreased the binding of A. fumigatus conidia to fibronectin, suggesting that oligosaccharides on fibronectin were not involved in conidiospore binding. Further evidence for this hypothesis came from experiments using purified fragments of fibronectin; A. fumigatus conidia preferentially bound to the nonglycosylated 40-kDa fragment which contains the glycosaminoglycan (GAG) binding domain. Negatively charged carbohydrates, including dextran sulfate and heparin, as well as high-ionic-strength buffers, inhibited binding of A. fumigatus conidia to both fibronectin and intact basal lamina, suggesting that negatively charged carbohydrates on the surface of the conidium may bind to the GAG binding domain of fibronectin and other basal-lamina proteins. These data provide evidence for a novel mechanism of conidial attachment whereby adherence to fibronectin and other basal-lamina proteins is mediated via negatively charged carbohydrates on the conidial surface.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.