ArticleInfection and immunity2000
Construction and characterization of Haemophilus ducreyi lipooligosaccharide (LOS) mutants defective in expression of heptosyltransferase III and beta1,4-glucosyltransferase: identification of LOS glycoforms containing lactosamine repeats.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 56 citations in OpenAlex.
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- Article
- The Glaesserella parasuis phosphoglucomutase is partially required for lipooligosaccharide synthesis.Veterinary research · 2020Article
- Characterization of a trifunctional glucosyltransferase essential for Moraxella catarrhalis lipooligosaccharide assembly.Glycobiology · 2013Article
- Structural characterization of an oligosaccharide made by Neisseria sicca.Journal of bacteriology · 2009Article
- Comparative proteomic analysis of the Haemophilus ducreyi porin-deficient mutant 35000HP::P2AB.Journal of bacteriology · 2009Article
- Genetic analysis of lipooligosaccharide core biosynthesis in Campylobacter jejuni 81-176.Journal of bacteriology · 2008Article
- Metabolic analysis of Moraxella catarrhalis and the effect of selected in vitro growth conditions on global gene expression.Infection and immunity · 2007Article
- Identification of genes involved in the expression of atypical lipooligosaccharide structures from a second class of Haemophilus ducreyi.Infection and immunity · 2007Article
- Identification of a D-glycero-D-manno-heptosyltransferase gene from Helicobacter pylori.Journal of bacteriology · 2005Article
- Characterization of a cluster of three glycosyltransferase enzymes essential for Moraxella catarrhalis lipooligosaccharide assembly.Journal of bacteriology · 2005Article
- A heptosyltransferase mutant of Pasteurella multocida produces a truncated lipopolysaccharide structure and is attenuated in virulence.Infection and immunity · 2004Article
- Genetic and structural characterization of the core region of the lipopolysaccharide from Serratia marcescens N28b (serovar O4).Journal of bacteriology · 2004Article
- Signature-tagged mutagenesis of Pasteurella multocida identifies mutants displaying differential virulence characteristics in mice and chickens.Infection and immunity · 2003Article
- Identification and characterization of the N-acetylglucosamine glycosyltransferase gene of Haemophilus ducreyi.Infection and immunity · 2002Article
- Development of a rapid immunodiagnostic test for Haemophilus ducreyi.Journal of clinical microbiology · 2002Article
- Characterization of lipooligosaccharides from Haemophilus ducreyi containing polylactosamine repeats.Journal of the American Society for Mass Spectrometry · 2002Article
- The lbgAB gene cluster of Haemophilus ducreyi encodes a beta-1,4-galactosyltransferase and an alpha-1,6-DD-heptosyltransferase involved in lipooligosaccharide biosynthesis.Infection and immunity · 2002Article
- Comparative and genetic analyses of the putative Vibrio cholerae lipopolysaccharide core oligosaccharide biosynthesis (wav) gene cluster.Infection and immunity · 2002Article
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
To begin to understand the role of the lipooligosaccharide (LOS) molecule in chancroid infections, we constructed mutants defective in expression of glycosyltransferase genes. Pyocin lysis and immunoscreening was used to identify a LOS mutant of Haemophilus ducreyi 35000. This mutant, HD35000R, produced a LOS molecule that lacked the monoclonal antibody 3F11 epitope and migrated with an increased mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Structural studies indicated that the principal LOS glycoform contains lipid A, Kdo, and two of the three core heptose residues. HD35000R was transformed with a plasmid library of H. ducreyi 35000 DNA, and a clone producing the wild-type LOS was identified. Sequence analysis of the plasmid insert revealed one open reading frame (ORF) that encodes a protein with homology to the WaaQ (heptosyltransferase III) of Escherichia coli. A second ORF had homology to the LgtF (glucosyltransferase) of Neisseria meningitidis. Individual isogenic mutants lacking expression of the putative H. ducreyi heptosyltransferase III, the putative glucosyltransferase, and both glycosyltransferases were constructed and characterized. Each mutant was complemented with the representative wild-type genes in trans to restore expression of parental LOS and confirm the function of each enzyme. Matrix-assisted laser desorption ionization mass spectrometry and SDS-PAGE analysis identified several unique LOS glycoforms containing di-, tri-, and poly-N-acetyllactosamine repeats added to the terminal region of the main LOS branch synthesized by the heptosyltransferase III mutant. These novel H. ducreyi mutants provide important tools for studying the regulation of LOS assembly and biosynthesis.
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