ArticleInfection and immunity2000
Intercellular communication in Helicobacter pylori: luxS is essential for the production of an extracellular signaling molecule.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 90 citations in OpenAlex.
- Vacuolating Cytotoxin A (VacA) and Extracellular Vesicles inIranian journal of pathology · 2025Review
- Helicobacter pylori biofilm interference by N-acyl homoserine lactonases: in vitro and in silico approaches.Molecular biology reports · 2024Article
- Essential role ofInfection and immunity · 2023Article
- Biofilm ofAntibiotics (Basel, Switzerland) · 2023Review
- Identification of an Essential LolD-Like Protein in Helicobacter pylori.Journal of bacteriology · 2023Article
- Gastric microbiota dysbiosis andFrontiers in microbiology · 2023Review
- AI-2 Induces Urease Expression Through Downregulation of Orphan Response Regulator HP1021 inFrontiers in medicine · 2022Article
- Quorum sensing inPeerJ · 2021Article
- Article
- Review
- Growth phase-dependent composition of the Helicobacter pylori exoproteome.Journal of proteomics · 2016Article
- From deep-sea volcanoes to human pathogens: a conserved quorum-sensing signal in Epsilonproteobacteria.The ISME journal · 2015Article
- Biofilm Formation by Helicobacter pylori and Its Involvement for Antibiotic Resistance.BioMed research international · 2015Review
- Control of gene expression in Helicobacter pylori using the Tet repressor.Journal of microbiological methods · 2013Article
- Quorum sensing dependent phenotypes and their molecular mechanisms in Campylobacterales.European journal of microbiology & immunology · 2012Review
- Helicobacter pylori perceives the quorum-sensing molecule AI-2 as a chemorepellent via the chemoreceptor TlpB.Microbiology (Reading, England) · 2011Article
- Methylthioadenosine/S-adenosylhomocysteine nucleosidase, a critical enzyme for bacterial metabolism.Molecular microbiology · 2011Review
- In Helicobacter pylori auto-inducer-2, but not LuxS/MccAB catalysed reverse transsulphuration, regulates motility through modulation of flagellar gene transcription.BMC microbiology · 2010Article
- Lack of genomic evidence of AI-2 receptors suggests a non-quorum sensing role for luxS in most bacteria.BMC microbiology · 2008Article
- Adherence of Helicobacter pylori to abiotic surfaces is influenced by serum.Applied and environmental microbiology · 2008Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Individual bacteria of numerous species can communicate and coordinate their actions via the production, release, and detection of extracellular signaling molecules. In this study, we used the Vibrio harveyi luminescence bioassay to determine whether Helicobacter pylori produces such a factor. Cell-free conditioned media from H. pylori strains 60190 and 26695 each induced >100-fold-greater luminescence in V. harveyi than did sterile culture medium. The H. pylori signaling molecule had a molecular mass of <10 kDa, and its activity was unaffected by heating to 80 degrees C for 5 min or protease treatment. The genome sequence of H. pylori 26695 does not contain any gene predicted to encode an acyl homoserine lactone synthase but does contain an orthologue of luxS, which is required for production of autoinducer-2 (AI-2) in V. harveyi. To evaluate the role of luxS in H. pylori, we constructed luxS null mutants derived from H. pylori 60190 and 26695. Conditioned media from the wild-type H. pylori strains induced >100-fold-greater luminescence in the V. harveyi bioassay than did conditioned medium from either mutant strain. Production of the signaling molecule was restored in an H. pylori luxS null mutant strain by complementation with a single intact copy of luxS placed in a heterologous site on the chromosome. In addition, Escherichia coli DH5alpha produced autoinducer activity following the introduction of an intact copy of luxS from H. pylori. Production of the signaling molecule by H. pylori was growth phase dependent, with maximal production occurring in the mid-exponential phase of growth. Transcription of H. pylori vacA also was growth phase dependent, but this phenomenon was not dependent on luxS activity. These data indicate that H. pylori produces an extracellular signaling molecule related to AI-2 from V. harveyi. We speculate that this signaling molecule may play a role in regulating H. pylori gene expression.
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