ArticleThe Biochemical journal2000
Interaction of Bacillus subtilis CsaA with SecA and precursor proteins.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 59 citations in OpenAlex.
- Protein trafficking across the cell envelope of gram-positive bacteria.Journal of bacteriology · 2025Review
- Engineering Bacillus subtilis for high-value bioproduction: recent advances and applications.Microbial cell factories · 2025Review
- Coexpressing the Signal Peptide of Vip3A and the Trigger Factor ofFrontiers in microbiology · 2022Article
- Update on the Protein Homeostasis Network inFrontiers in microbiology · 2022Review
- Progress in research and application development of surface display technology using Bacillus subtilis spores.Applied microbiology and biotechnology · 2020Review
- Article
- Bottleneck in secretion of α-amylase in Bacillus subtilis.Microbial cell factories · 2017Review
- Breaking the bacterial protein targeting and translocation model: oral organisms as a case in point.Molecular oral microbiology · 2015Review
- Article
- Molecular engineering of secretory machinery components for high-level secretion of proteins in Bacillus species.Journal of industrial microbiology & biotechnology · 2014Review
- Sec-secretion and sortase-mediated anchoring of proteins in Gram-positive bacteria.Biochimica et biophysica acta · 2014Review
- Structural characterization of the complex of SecB and metallothionein-labeled proOmpA by cryo-electron microscopy.PloS one · 2012Article
- Article
- Towards the development of Bacillus subtilis as a cell factory for membrane proteins and protein complexes.Microbial cell factories · 2008Article
- Mapping the pathways to staphylococcal pathogenesis by comparative secretomics.Microbiology and molecular biology reviews : MMBR · 2006Review
- Contribution of the alanine-rich region of Streptococcus mutans P1 to antigenicity, surface expression, and interaction with the proline-rich repeat domain.Infection and immunity · 2004Article
- The archaeal Sec-dependent protein translocation pathway.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2004Review
- Proteomics of protein secretion by Bacillus subtilis: separating the "secrets" of the secretome.Microbiology and molecular biology reviews : MMBR · 2004Review
- The crystal structure of the ttCsaA protein: an export-related chaperone from Thermus thermophilus.The EMBO journal · 2001Article
- Structure of the EMAPII domain of human aminoacyl-tRNA synthetase complex reveals evolutionary dimer mimicry.The EMBO journal · 2001Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CsaA from the Gram-positive bacterium Bacillus subtilis has been identified previously as a suppressor of the growth and protein-export defect of Escherichia coli secA(Ts) mutants. CsaA has chaperone-like activities in vivo and in vitro. To examine the role of CsaA in protein export in B. subtilis, expression of the csaA gene was repressed. While export of most proteins remained unaffected, export of at least two proteins was significantly reduced upon CsaA depletion. CsaA co-immunoprecipitates and co-purifies with the SecA proteins of E. coli and B. subtilis, and binds the B. subtilis preprotein prePhoB. Purified CsaA stimulates the translocation of prePhoB into E. coli membrane vesicles bearing the B. subtilis translocase, whereas it interferes with the SecB-mediated translocation of proOmpA into membrane vesicles of E. coli. The specific interaction with the SecA translocation ATPase and preproteins suggests that CsaA acts as a chaperone that promotes the export of a subset of preproteins in B. subtilis.
Indexed as
Identifiers
10816431PMC1221075W2123351983What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.