ArticleVirology2000
Characterization of murine coronavirus neutralization epitopes with phage-displayed peptides.
Article in Virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- The development of inovirus-associated vector vaccines using phage-display technologies.Expert review of vaccines · 2019Review
- Putative phage-display epitopes of the porcine epidemic diarrhea virus S1 protein and their anti-viral activity.Virus genes · 2015Article
- Beyond phage display: non-traditional applications of the filamentous bacteriophage as a vaccine carrier, therapeutic biologic, and bioconjugation scaffold.Frontiers in microbiology · 2015Review
- Review
- Requirements for empirical immunogenicity trials, rather than structure-based design, for developing an effective HIV vaccine.Archives of virology · 2012Review
- A general insert label for peptide display on chimeric filamentous bacteriophages.Analytical biochemistry · 2012Article
- Identification and characterization of a neutralizing-epitope-containing spike protein fragment in turkey coronavirus.Archives of virology · 2011Article
- Exploring peptide mimics for the production of antibodies against discontinuous protein epitopes.Molecular immunology · 2010Article
- Structure of a high-affinity "mimotope" peptide bound to HIV-1-neutralizing antibody b12 explains its inability to elicit gp120 cross-reactive antibodies.Journal of molecular biology · 2007Article
- Screening random peptide libraries with subacute sclerosing panencephalitis brain-derived recombinant antibodies identifies multiple epitopes in the C-terminal region of the measles virus nucleocapsid protein.Journal of virology · 2006Article
- Phage-displayed peptides having antigenic similarities with porcine epidemic diarrhea virus (PEDV) neutralizing epitopes.Virology · 2006Article
- Identification of one peptide which inhibited infectivity of avian infectious bronchitis virus in vitro.Science in China. Series C, Life sciences · 2006Article
- A human SARS-CoV neutralizing antibody against epitope on S2 protein.Biochemical and biophysical research communications · 2005Article
- Identification of an antigenic determinant on the S2 domain of the severe acute respiratory syndrome coronavirus spike glycoprotein capable of inducing neutralizing antibodies.Journal of virology · 2004Article
- Identifying epitopes of HIV-1 that induce protective antibodies.Nature reviews. Immunology · 2004Review
- Random-peptide libraries and antigen-fragment libraries for epitope mapping and the development of vaccines and diagnostics.Current opinion in chemical biology · 2001Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Phage-displayed peptide libraries were used to map immunologically relevant epitopes on the surface (S) glycoprotein of a neurotropic murine coronavirus (MHV-A59). Three in vitro virus-neutralizing and in vivo protective mAbs against either continuous or discontinuous epitopes on the S glycoprotein were used to screen 12 different peptide libraries expressed on the pVIII major coat protein of the fd filamentous bacteriophage. Consensus sequences that matched short sequences within the S glycoprotein were identified. The sequence of a tight-binding, mAb-selected peptide suggested the location of a discontinuous epitope within the N-terminal S1 subunit. Several tightly binding phage were amplified and used directly as immunogens in BALB/c and C57BL/6 mice. Partial protection of C57BL/6 mice against a lethal acute virus infection was achieved with a phage preparation that displayed a linear epitope. Protection correlated with the presence of sufficient levels of specific antiviral antibodies recognizing the same immunodominant domain and 13-mer peptide, located within the C-terminal S2 subunit, as the selecting mAb. Thus, the direct use of phage-displayed peptides to evaluate protective antiviral immune responses complements their use to characterize antibody-binding epitopes. This is the first evaluation of protective immunization induced by mAb-selected phage-displayed peptides.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.