ArticleThe Journal of clinical investigation2000
Osmotic water permeabilities of cultured, well-differentiated normal and cystic fibrosis airway epithelia.
Article in The Journal of clinical investigation, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
79 citing papers in PubMed, 184 citations in OpenAlex.
- Hypertonic saline has a prolonged effect on mucociliary clearance in adults with cystic fibrosis.Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society · 2018Trial
- Epithelial Sodium Channel in the Respiratory System: A Bibliometric Review of Recent Studies.Biology · 2026Review
- Transient receptor potential vanilloid 4 channels contribute to the initiation of water-induced swallowing reflexes.Scientific reports · 2026Article
- Transient receptor potential vanilloid 4 channels contribute to the initiation of water-induced swallowing reflexes.Research square · 2026Article
- Polymer solutions under steady solvent flow between two semipermeable interfaces.Macromolecules · 2025Article
- Do pulmonary ionocytes absorb chloride or secrete chloride?American journal of physiology. Cell physiology · 2025Review
- Increased ENaC-mediated liquid absorption across vitamin-D deficient human airway epithelia.American journal of physiology. Cell physiology · 2024Article
- Understanding Bidirectional Water Transport across Bronchial Epithelial Cell Monolayers: A Microfluidic Approach.Membranes · 2023Article
- CFTR-rich ionocytes mediate chloride absorption across airway epithelia.The Journal of clinical investigation · 2023Article
- Impact of high- and low-flow nebulised saline on airway hydration and mucociliary transport.ERJ open research · 2023Article
- Mouth breathing, dry air, and low water permeation promote inflammation, and activate neural pathways, by osmotic stresses acting on airway lining mucus.QRB discovery · 2023Article
- Mucociliary clearance affected by mucus-periciliary interface stimulations using analytical solution during cough and sneeze.European physical journal plus · 2023Article
- The effect of Ramadan fasting on nasal mucociliary activity and peak nasal inspiratory flow.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2022Article
- Physiology and pathophysiology of human airway mucus.Physiological reviews · 2022Review
- FXYD3 facilitates NaAmerican journal of physiology. Cell physiology · 2022Article
- RNA sequencing identifies transcriptional changes in the rabbit larynx in response to low humidity challenge.BMC genomics · 2020Article
- A physiologically-motivated model of cystic fibrosis liquid and solute transport dynamics across primary human nasal epithelia.Journal of pharmacokinetics and pharmacodynamics · 2019Article
- Pathological mucus and impaired mucus clearance in cystic fibrosis patients result from increased concentration, not altered pH.The European respiratory journal · 2018Article
- CrossTalk proposal: mucosal acidification drives early progressive lung disease in cystic fibrosis.The Journal of physiology · 2018Article
- An integrated mathematical epithelial cell model for airway surface liquid regulation by mechanical forces.Journal of theoretical biology · 2018Article
19 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on byAirway plumbing.2000
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Current hypotheses describing the function of normal airway surface liquid (ASL) in lung defense are divergent. One theory predicts that normal airways regulate ASL volume by modulating the flow of isosmotic fluid across the epithelium, whereas an alternative theory predicts that ASL is normally hyposmotic. These hypotheses predict different values for the osmotic water permeability (P(f)) of airway epithelia. We measured P(f) of cultures of normal and cystic fibrosis (CF) airway epithelia that, like the native tissue, contain columnar cells facing the lumen and basal cells that face a basement membrane. Xz laser scanning confocal microscopy recorded changes in epithelial height and transepithelial volume flow in response to anisosmotic challenges. With luminal hyperosmotic challenges, transepithelial and apical membrane P(f) are relatively high for both normal and CF airway epithelia, consistent with an isosmotic ASL. Simultaneous measurements of epithelial cell volume and transepithelial water flow revealed that airway columnar epithelial cells behave as osmometers whose volume is controlled by luminal osmolality. Basal cell volume did not change in these experiments. When the serosal side of the epithelium was challenged with hyperosmotic solutions, the basal cells shrank, whereas the lumen-facing columnar cells did not. We conclude that (a) normal and CF airway epithelia have relatively high water permeabilities, consistent with the isosmotic ASL theory, and the capacity to restore water on airway surfaces lost by evaporation, and (b) the columnar cell basolateral membrane and tight junctions limit transepithelial water flow in this tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.